Simultaneous measurement of S, macronutrients, and heavy metals in the soil microbial biomass with CHCl3 fumigation and NH4NO3 extraction
Simultaneous measurement of S, macronutrients, and heavy metals in the soil microbial biomass with CHCl3 fumigation and NH4NO3 extraction
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DOI：
10.1016/j.soilbio.2008.11.001
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发表时间：
2009-02-01
影响因子：
9.7
通讯作者：
Joergensen, Rainer Georg
中科院分区：
文献类型：
--
作者：
Khan, Khalid Saifullah;Heinze, Stefanie;Joergensen, Rainer Georg
研究了1MNH4NO3提取法是否可以替代10mMCaCl2提取法来估算土壤微生物量S，以及CHCl3不稳定的NH4NO3可提取的常量营养元素和重金属的数据是否有用，并与现有的土壤微生物元素浓度数据相一致。微生物量C紧随其后的是提取CaCl2后的微生物量S，平均C/S比为82；提取硝酸铵后的微生物量S，平均C/S比为57。未熏蒸土壤中CHCl3活性金属对NH4NO3可提取态的平均贡献率为0.1%~112%，大小顺序为钾<；镁<；镉<；钠；锌+镍<；锰和铜。CHCl3活性金属对微生物生物量C的平均贡献率为0.03-22ppm，大小顺序为镉、镍、锌、锰、镁、铜、钠、钾。在不同的金属中，这些相对贡献从4倍(Na+)到200多倍(Cu-2)不等。CHCl3活性锌、钠，尤其是钾与微生物量C呈显著正相关。除铜以外的所有元素与微生物生物量C的关系都在有限的文献中已知的范围内，这些真菌生长在重金属污染的土壤上。(C)2008爱思唯尔有限公司。保留所有权利。
The study was carried out to investigate whether 1 M NH4NO3 extraction is a useful alternative to 10 mM CaCl2 extraction for estimating soil microbial biomass S and whether the data of CHCl3-labile NH4NO3-extractable macronutrients and heavy metals are useful and in agreement with the available data on element concentrations in soil microorganisms. Microbial biomass C was followed by microbial biomass S after CaCl2 extraction with an average C/S ratio of 82, and by microbial biomass S after NH4NO3 extraction with an average C/S ratio of 57. The mean contribution of CHCl3-labile metals in relation to the NH4NO3-extractable fraction from non-fumigated soils ranged from 0.1 to 112% in the order potassium < magnesium < cadmium < sodium < zinc + nickel < manganese < copper. The mean contribution of CHCl3-labile metals in relation to the microbial biomass C ranged from 0.03 to 22 parts per thousand in the order cadmium < nickel < zinc < manganese < magnesium < copper < sodium < potassium. These relative contributions varied within the different metals from a 4-fold (Na+) to a more than 200-fold range (Cu-2). Significant positive correlations with microbial biomass C were observed for CHCl3-labile zinc, sodium and especially potassium. The concentration of all elements except copper in relation to microbial biomass C were in the range known from the limited literature on fungi grown on heavy metal contaminated soils. (C) 2008 Elsevier Ltd. All rights reserved.