Proteomic Analysis of a Poplar Cell Suspension Culture Suggests a Major Role of Protein S-Acylation in Diverse Cellular Processes.
Proteomic Analysis of a Poplar Cell Suspension Culture Suggests a Major Role of Protein S-Acylation in Diverse Cellular Processes.
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卵形家族蛋白概述，一类新型植物特异性生长调节剂
DOI：
10.3389/fpls.2016.00477
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发表时间：
2016
影响因子：
5.6
通讯作者：
Bulone V
中科院分区：
文献类型：
--
作者：
Srivastava V;Weber JR;Malm E;Fouke BW;Bulone V
S-酰化是蛋白质的可逆翻译后修饰，已知其参与膜靶向、亚细胞运输和蛋白质的多种功能性质的确定。本工作的目的是确定白杨S-酰化蛋白。使用的酰基-生物素交换方法和质谱允许的约450 S-酰化的蛋白质，这被细分为三个主要的蛋白质组参与运输，信号转导和应激反应，分别进行鉴定。最大的S-酰化蛋白质组是蛋白激酶超家族。可溶性N-乙基马来酰亚胺敏感因子激活蛋白受体，带7家族蛋白和四跨膜蛋白，所有主要涉及细胞内运输，也被确定。此外，细胞壁相关蛋白质，包括纤维素酶和其他葡聚糖酶，被发现是S-酰化。24个确定的S-酰化蛋白质也富含洗涤剂抗性膜微区，这表明S-酰化在蛋白质定位到专门的质膜亚区中起着关键作用。该数据集有望增强我们目前对植物中S-酰化蛋白质的各种功能的理解。
S-acylation is a reversible post-translational modification of proteins known to be involved in membrane targeting, subcellular trafficking, and the determination of a great variety of functional properties of proteins. The aim of this work was to identify S-acylated proteins in poplar. The use of an acyl-biotin exchange method and mass spectrometry allowed the identification of around 450 S-acylated proteins, which were subdivided into three major groups of proteins involved in transport, signal transduction, and response to stress, respectively. The largest group of S-acylated proteins was the protein kinase superfamily. Soluble N-ethylmaleimide-sensitive factor-activating protein receptors, band 7 family proteins and tetraspanins, all primarily related to intracellular trafficking, were also identified. In addition, cell wall related proteins, including cellulose synthases and other glucan synthases, were found to be S-acylated. Twenty four of the identified S-acylated proteins were also enriched in detergent-resistant membrane microdomains, suggesting S-acylation plays a key role in the localization of proteins to specialized plasma membrane subdomains. This dataset promises to enhance our current understanding of the various functions of S-acylated proteins in plants.