Preferential Localization of Neural Cell Recognition Molecule NB-2 in Developing Glutamatergic Neurons in the Rat Auditory Brainstem
Preferential Localization of Neural Cell Recognition Molecule NB-2 in Developing Glutamatergic Neurons in the Rat Auditory Brainstem
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DOI：
10.1002/cne.21972
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发表时间：
2009-04-01
影响因子：
2.5
通讯作者：
Watanabe, Kazutada
中科院分区：
文献类型：
--
作者：
Toyoshima, Manabu;Sakurai, Kunie;Watanabe, Kazutada
NB-2是优先在听觉通路中表达的神经元细胞识别分子。NB-2基因缺陷的小鼠表现出对声刺激的异常反应。在这里，我们研究了NB-2的表达和定位在大鼠听觉脑干发育过程中。NB-2在耳蜗腹侧核（VCN）、腹侧听纹、外侧和内侧上级橄榄复合体（SOC）、上级橄榄旁核、斜方体内侧核（MNTB）、腹外侧丘系和下丘中央核（CIC）中表达较强。在VCN和CIC中，NB-2在已知对高频率响应的区域中表达。原位杂交结合免疫组化显示NB-2仅在神经元中表达。NB-2共定位于神经元和赫尔德的肾盏，但不与甘氨酸或GABA能元素中的谷氨酸能元素。NB-2在SOC中的表达在胚胎第19天（E）首次检测到，在出生后第7天（P）达到最高水平，此后下降。VGLUT 1和VGLUT 2分别是成熟和未成熟突触的标记物，与NB-2免疫标记结合显示NB-2在突触中表达。总的来说，我们的研究结果表明，NB-2在听觉发育的最后阶段，在脑干中的神经元能突触的成熟中起着关键作用。J. Comp.神经元513：349-362，2009. (C)2009 Wiley-Liss，Inc.
NB-2 is a neuronal cell recognition molecule that is preferentially expressed in auditory pathways. Mice deficient in the NB-2 gene exhibit aberrant responses to acoustic stimuli. Here we examined the expression and localization of NB-2 in the auditory brainstem during development in the rat. NB-2 was strongly expressed in the ventral cochlear nucleus (VCN), ventral acoustic stria, lateral and medial superior olivary complex (SOC), superior paraolivary nucleus, medial nucleus of the trapezoid body (MNTB), ventrolateral lemniscus, and central nucleus of the inferior colliculus (CIC). In the VCN and CIC, NB-2 was expressed in the regions that are known to respond to high frequencies. In situ hybridization combined with immunohistochemistry suggested that NB-2 is expressed only in neurons. NB-2 was colocalized with glutamatergic elements in the neuropil and the calyces of Held but not with glycinergic or GABAergic elements. NB-2 expression in the SOC was first detected at embryonic day (E)19, reached a maximum level at postnatal day (P)7, and declined thereafter. Immunolabeling with VGLUT1 and VGLUT2, markers for mature and premature glutamatergic synapses, respectively, in combination with NB-2 immunolabeling revealed that NB-2 is expressed at glutamatergic synapses. Collectively, our findings suggest that NB-2 plays a key role in maturation of glutamatergic synapses in the brainstem during the final stages of auditory development. J. Comp. Neurol. 513:349-362, 2009. (C) 2009 Wiley-Liss, Inc.