C3D,G DEPOSITS IN INFLAMMATORY SKIN DISEASES - USE OF PSORIATIC SKIN AS A MODEL OF CUTANEOUS INFLAMMATION
C3D,G DEPOSITS IN INFLAMMATORY SKIN DISEASES - USE OF PSORIATIC SKIN AS A MODEL OF CUTANEOUS INFLAMMATION
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DOI：
10.1111/1523-1747.ep12371702
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发表时间：
1993-12-01
影响因子：
6.5
通讯作者：
GUILHOU, JJ
中科院分区：
文献类型：
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作者：
BASSETSEGUIN, N;PORNEUF, M;GUILHOU, JJ
我们实验室最近的研究表明，人角质形成细胞合成和分泌包括C3在内的补体成分。此外，人角化细胞来源的C3被认为是C3d，g的潜在来源，C3d，g是最近描述的正常表皮基底膜层下致密区成分。此外，人角化细胞来源的C3也可能导致自身免疫性或炎症性皮肤病中C3的表皮基底膜沉积。为了进一步了解正常和病变皮肤表皮基底膜C3沉积的特异性和起源，我们对各种炎症性皮肤病中C3和C3裂解片段的原位沉积进行了表征，并利用皮肤等效模型评估了正常或病变皮肤表皮外生物中新基底膜中C3裂解片段的沉积。在所有炎症皮肤样本中，C3d，g的反应性都更大，并且通常与这些部位的C3c反应性有关。未检测到免疫球蛋白或其他补体成分。将富含表皮基底膜C3c的病变性银屑病皮肤用于我们的器官培养系统，观察到C3在新基底膜内的掺入。这些结果表明，人角化细胞来源的C3可能有助于皮肤的炎症反应，并解释了正常表皮基底膜中C3d，g的沉积。
Recent studies in our laboratories have shown that human keratinocytes synthesize and secrete complement components including C3. Moreover, human keratinocyte-derived C3 is regarded as a potential source of C3d,g, a recently described constituent of the sublamina densa region of normal epidermal basement membrane. Additionally, human keratinocyte-derived C3 may also contribute to epidermal basement membrane deposits of C3 in autoimmune or inflammatory skin disorders. To further our understanding of the specificity and origin of epidermal basement membrane C3 deposits in normal and diseased skin, we have characterized in situ deposits of C3 and C3 cleavage fragments in various inflammatory skin diseases and utilized a skin equivalent model to assess the deposition of C3 cleavage fragments in neo-basement membrane of epidermal outgrowths from normal or diseased human skin. C3d,g reactivity was found to be greater in all samples of inflamed skin, and typically associated with C3c reactivity at these sites. No immunoglobulins or other complement components were detected. When lesional psoriatic skin rich in epidermal basement membrane C3c was used in our organ culture system, C3 incorporation within neo-basement membrane was observed. These results show that human keratinocyte-derived C3 may contribute to inflammatory reactions in skin as well as account for deposits of C3d,g in normal epidermal basement membrane.