Reciprocal connections between subdivisions of the dorsal raphe and the nuclear core of the locus coeruleus in the rat
Reciprocal connections between subdivisions of the dorsal raphe and the nuclear core of the locus coeruleus in the rat
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DOI：
10.1016/j.brainres.2004.08.022
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发表时间：
2004-11-05
期刊：
影响因子：
2.9
通讯作者：
Waterhouse, BD
中科院分区：
文献类型：
--
作者：
Kim, MA;Lee, HS;Waterhouse, BD
采用逆行示踪和免疫细胞化学方法，对大鼠脑干单胺能核中缝背核（dorsal raphe， DR）和蓝斑核（locus coeruleus， LC）之间的相互作用进行了分析。将金偶联和灭活的小麦胚芽凝集素-辣根过氧化物酶（WGA-apo-HRP-gold）注射到LC核核心的DR或rostro-尾侧水平，分别用多巴胺- β -羟化酶（DBH）或5-羟色胺（5-HT）免疫染色鉴定标记的LC或DR神经元。在LC-DR投射中，尾侧主LC投射到尾侧、腹内侧和束间DR。中LC和尾侧LC以同侧优势投射到DR的侧翼分支。少数吻侧LC神经元投射到尾侧、背内侧和腹内侧DR。在DR-LC投射中，吻侧LC主要接收来自尾侧、背内侧和腹内侧DR的输入。中LC到尾侧LC接收来自中DR到尾侧DR的投射。与同侧侧翼和尾侧DR相比，DR LC的投影更坚固，并且包含了5 -羟色胺能和非5 -羟色胺能成分。因此，数据显示了DR和LC之间的地形有序，相互连接，从DR到LC的投影特别强。这两个脑干单胺能核之间的交流可能对多种功能至关重要，包括睡眠-觉醒调节、警觉性、镇痛、认知和应激反应。(C) 2004 Elsevier B.V.版权所有
The interconnection between two brainstem monoaminergic nuclei, the dorsal raphe (DR) and the locus coeruleus (LC), was analyzed in the rat using retrograde tracing and immunocytochemistry. Gold-conjugated and inactivated wheatgerm agglutinin-horseradish peroxidase (WGA-apo-HRP-gold) was injected into subdivisions of the DR or rostro-caudal levels of the nuclear core of the LC, and labeled LC or DR neurons were identified by dopamine-beta-hydroxylase (DBH) or 5-hydroxytryptamine (5-HT) immunostaining, respectively. Within the LC-DR projection, the caudal principal LC projected to the caudal, ventromedial, and interfascicular DR. Mid-LC as well as caudal LC projected with an ipsilateral predominance to the lateral wing subdivision of the DR. A few rostral LC neurons projected to caudal, dorsomedial, and ventromedial DR. Within the DR-LC projection, the rostral LC received inputs mainly from the caudal, dorsomedial, and ventromedial DR. Mid-LC to caudal LC received projections from mid-DR to caudal DR, with the heaviest projection from the ipsilateral lateral wing as well as caudal DR. The DR LC projection was substantially more robust than LC DR and included both serotonergic and nonserotonergic components. Thus, the data demonstrate topographically ordered, reciprocal connectivity between DR and LC with particularly strong projections from DR to LC. Communication between these two brainstem monoaminergic nuclei may be critical for a variety of functions including sleep-wake regulation, vigilance, analgesia, cognition, and stress responses. (C) 2004 Elsevier B.V. All rights reserved.