Major changes in the cell wall during silique development in Arabidopsis thaliana
Major changes in the cell wall during silique development in Arabidopsis thaliana
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DOI：
10.1016/j.phytochem.2010.10.008
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发表时间：
2011-01-01
期刊：
影响因子：
3.8
通讯作者：
Pelloux, Jerome
中科院分区：
文献类型：
--
作者：
Louvet, Romain;Rayon, Catherine;Pelloux, Jerome
果实发育是一个高度复杂的过程，涉及植物代谢的重大变化，导致细胞的生长和分化。细胞壁组成和结构的变化在调节细胞生长中起着重要作用。研究了模式植物拟南芥（Arabidopsis thaliana）干果发育过程中细胞壁组成及相关酶活性的变化。硅酸发育的特点是有几个特定的阶段，导致果实开裂和种子传播。我们发现，硅酸生长的早期阶段以非纤维素糖含量（鼠李糖、阿拉伯糖、木糖、半乳糖和半乳糖醛酸）的特定变化为特征。木葡聚糖低聚糖质量谱进一步显示，在硅酸发育过程中，o -乙酰化木葡聚糖的含量显著增加，这可能表明木葡聚糖相互结合或与纤维素结合的能力下降。由果胶甲基酯酶（PMEs）活性介导的甲基化程度在果胶生长和开裂过程中下降。我们的分析揭示了细胞壁组成的主要变化，表明它可能是调节细胞壁流变导致生长或生长停滞的主要决定因素。(C) 2010 Elsevier Ltd.版权所有。
Fruit development is a highly complex process, which involves major changes in plant metabolism leading to cell growth and differentiation. Changes in cell wall composition and structure play a major role in modulating cell growth. We investigated the changes in cell wall composition and the activities of associated enzymes during the dry fruit development of the model plant Arabidopsis thaliana. Silique development is characterized by several specific phases leading to fruit dehiscence and seed dispersal. We showed that early phases of silique growth were characterized by specific changes in non-cellulosic sugar content (rhamnose, arabinose, xylose, galactose and galacturonic acid). Xyloglucan oligosaccharide mass profiling further showed a strong increase in O-acetylated xyloglucans over the course of silique development, which could suggest a decreased capacity of xyloglucans to be associated with each other or to cellulose. The degree of methylesterification, mediated by the activity of pectin methylesterases (PMEs), decreased over the course of silique growth and dehiscence. The major changes in cell wall composition revealed by our analysis suggest that it could be major determinants in modulating cell wall rheology leading to growth or growth arrest. (C) 2010 Elsevier Ltd. All rights reserved.