A 3D-Printable Dual Beam Spectrophotometer with Multiplatform Smartphone Adaptor
A 3D-Printable Dual Beam Spectrophotometer with Multiplatform Smartphone Adaptor
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DOI：
10.1021/acs.jchemed.8b00870
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发表时间：
2019-07-01
影响因子：
3
通讯作者：
Smith, Adam W.
中科院分区：
文献类型：
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作者：
Bogucki, Ryan;Greggila, Mary;Smith, Adam W.
低成本、开源的分析仪器有可能提高学生的教育成果，并使大规模的公民科学项目成为可能。这些仪器中的许多都依赖智能手机来收集数据，主要是因为它们可以有效地利用光学传感器的巨大性价比。然而，要使这些设备得到更广泛的应用，还需要克服几个障碍。在这篇文章中，我们将重点介绍可见光分光光度计，它在化学实验室中很常见，因为每天都需要定量浓度。为了使基于srartphone的光谱仪更广泛地应用，我们设计了一种具有双光束光学几何形状的3d打印分光光度计。这种几何结构允许在同一张照片上收集样品和参考数据，从而提高了光谱的信噪比和再现性。还开发了一个通用安装系统，以允许各种智能手机外形因素耦合到分光光度计。为了证明该装置的潜在应用，报告了两项分析。第一个是用普通的家用染料简单说明比尔-朗伯定律。第二种是硝酸盐比色法，它显示了吸收和硝酸盐浓度之间的定量关系。动力学数据也显示了硝酸盐分析，这说明了从该装置获得的光谱数据的长时间稳定性。
Low cost, open-source analytical instrumentation has the potential to increase educational outcomes for students and enable large-scale citizen science projects. Many of these instruments rely on smartphones to collect the data, mainly because they can effectively leverage a dramatic price-to-performance ratio of the optical sensors. However, several hurdles need to be overcome for these devices to he more widely adapted. In this communication we focus on visible spectrophotometers, which are common in chemistry laboratories because of the day-to-day need for quantifying concentration. To make srnartphone-based spectrometers practical for wider use, we have designed a 3D-printable spectrophotometer with a dual-beam optical geometry. This geometry allows for sample and reference data to be collected on the same photograph and thus improves the signal-to-noise ratio and reproducibility of the spectra. A universal mounting system was also developed to allow for a wide variety of smartphone form factors to be coupled to the spectrophotometer. To demonstrate potential applications of this device, two assays are reported. The first is a simple illustration of the Beer-Lambert Law with common household dyes. The second is a colorimetric nitrate assay, which shows a quantitative relationship between absorption and nitrate concentration. Kinetic data are also shown for the nitrate assay, which illustrate the long time-stability of the spectral data acquired from the device.