Tracking Adventitial Fibroblast Contribution to Disease: A Review of Current Methods to Identify Resident Fibroblasts.
Tracking Adventitial Fibroblast Contribution to Disease: A Review of Current Methods to Identify Resident Fibroblasts.
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DOI：
10.1161/atvbaha.117.308199
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发表时间：
2017-09
期刊：
影响因子：
--
通讯作者：
Tallquist MD
中科院分区：
文献类型：
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作者：
Kuwabara JT;Tallquist MD
存在于血管外膜或血管最外层的细胞有助于血管疾病的进展，例如动脉粥样硬化、高血压和主动脉夹层。主动脉的外膜成纤维细胞是原型血管周围成纤维细胞，但外膜由多个不同的细胞群组成。因此，唯一识别成纤维细胞的方法对于更好地理解这些细胞如何促进疾病过程至关重要。区分外膜细胞类型的流行方法依赖于在小鼠中使用遗传工具来追踪和操纵这些细胞。随着报告基因和Cre重组酶表达小鼠在血管疾病研究中的使用越来越频繁，概述这些遗传工具的优点和局限性非常重要。这篇综述的目的是提供一个概述的各种遗传工具，可在小鼠的研究居民外膜成纤维细胞。
Cells present in the adventitia, or outermost layer of the blood vessel, contribute to the progression of vascular diseases, such as atherosclerosis, hypertension, and aortic dissection. The adventitial fibroblast of the aorta is the prototypic perivascular fibroblast, but the adventitia is composed of multiple distinct cell populations. Therefore, methods for uniquely identifying the fibroblast are critical for a better understanding of how these cells contribute to disease processes. A popular method for distinguishing adventitial cell types relies on the use of genetic tools in the mouse to trace and manipulate these cells. As reporter and Cre recombinase expressing mice are used more frequently in studies of vascular disease, it is important to outline the advantages and limitations of these genetic tools. The purpose of this review is to provide an overview of the various genetic tools available in the mouse for the study of resident adventitial fibroblasts.