Effect of adenine on liver nucleotide after fructose loading by 31P-NMR.
Effect of adenine on liver nucleotide after fructose loading by 31P-NMR.
复制标题
通过 31P-NMR 检测果糖负载后腺嘌呤对肝核苷酸的影响。
DOI：
10.1152/ajpgi.1989.256.6.g949
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Ugurbil,K
中科院分区：
文献类型：
--
作者：
Thoma,WJ;Ugurbil,K
125 ~ 150 g大鼠肝脏灌注，采用31P核磁共振（NMR）观察果糖代谢对肝脏能量的影响。肝脏以非再循环方式暴露于果糖中约15分钟，并监测30分钟的恢复情况。在1至25毫米的浓度范围内，果糖暴露导致三磷酸核苷水平的消耗。随后，果糖浓度为1 mM时，三磷酸核苷水平完全恢复；然而，当果糖浓度为10毫米时，回收率仅为初始值的65%，当浓度为25毫米时，回收率为55%。研究发现，通过阻断AMP的脱氨作用，腺嘌呤可以保护三磷酸核苷水平免受果糖的不利影响。在腺嘌呤存在的情况下，肝脏暴露于果糖时，在核磁共振光谱中观察到AMP的积累。
Livers from 125- to 150-g rats were perfused and the effect of fructose metabolism on liver energetics was observed using 31P nuclear magnetic resonance (NMR). Livers were exposed to fructose in a nonrecirculating manner for approximately 15 min and monitored for recovery for 30 min. At concentrations ranging from 1 to 25 mM, fructose exposure led to depletion of the nucleoside triphosphate level. Subsequently, the nucleoside triphosphate level recovered fully at a fructose concentration of 1 mM; however, recovery was only 65% of the initial values if the fructose concentration was 10 mM and 55% if the concentration was 25 mM. Adenine was found to protect nucleoside triphosphate levels against the adverse effects of fructose by blocking the deamination of AMP. In the presence of adenine, AMP accumulation was observed in the NMR spectra upon exposure of the liver to fructose.