REACTION OF TETRAHYDROFOLIC ACID WITH CYANATE FROM UREA SOLUTIONS - FORMATION OF AN INACTIVE FOLATE DERIVATIVE
REACTION OF TETRAHYDROFOLIC ACID WITH CYANATE FROM UREA SOLUTIONS - FORMATION OF AN INACTIVE FOLATE DERIVATIVE
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DOI：
10.1093/ajcn/30.12.2028
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发表时间：
1977-01-01
影响因子：
7.1
通讯作者：
KRUMDIECK, CL
中科院分区：
文献类型：
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作者：
FRANCIS, KT;THOMPSON, RW;KRUMDIECK, CL
在6 M尿素中制备的鹌鹑肝提取物比在1%抗坏血酸中制备的提取物含有更少的微生物可检测叶酸。研究了尿素溶液中氰酸盐与无活性叶酸衍生物反应的可能性。双标记的2-14C， 3”，5”，9(n)-3H四氢叶酸与氰酸钠在H下反应得到的化合物，在酸化去除过量的氰酸盐后，在285 nm处显示出单一的紫外吸收峰。原料和成品的14C/3H比相同。14C尿素与非放射性四氢叶酸反应得到14C标记衍生物。该化合物不支持干酪乳杆菌的生长。在氰酸盐或硫氰酸盐治疗的患者和尿毒症患者中叶酸失活可能具有临床意义。
Quail liver extracts prepared in 6 M urea contain significantly less microbiologically assayable folates than extracts prepared in hot 1% ascorbate. The possibility that inactive folate derivatives are formed by reaction with the cyanate present in the urea solution was investigated. Doubly labeled 2-14C, 3'',5'',9(n)-3H tetrahydrofolic acid reacted under H with sodium cyanate gave a compound which, after acidification to remove excess cyanate, shows a single UV absorbing peak at 285 nm. The 14C/3H ratios of starting material and product were the same. Reaction of 14C urea with nonradioactive tetrahydrofolic acid yielded the 14C labeled derivative. The compound does not support Lactobacillus casei growth. Folate inactivation in cyanate or thiocyanate treated patients and in uremic patients may have clinical significance.