TRYPANOSOMA-CRUZI - MONOCLONAL-ANTIBODY TO CYTOSKELETON RECOGNIZES GIANT PROTEINS OF THE FLAGELLAR ATTACHMENT ZONE
TRYPANOSOMA-CRUZI - MONOCLONAL-ANTIBODY TO CYTOSKELETON RECOGNIZES GIANT PROTEINS OF THE FLAGELLAR ATTACHMENT ZONE
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DOI：
10.1006/expr.1995.1076
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发表时间：
1995-06-01
影响因子：
2.1
通讯作者：
COLLI, W
中科院分区：
文献类型：
--
作者：
RUIZMORENO, L;BIJOVSKY, AT;COLLI, W
克氏锥虫锥鞭毛体和外鞭毛体的细胞骨架制剂含有由单克隆抗体（2G 4）识别的蛋白质，该蛋白质连接到寄生虫两个阶段的鞭毛附着区。Western印迹分析显示，该抗体能够识别分子量高于700 kDa至2500 kDa的蛋白条带。这些巨大的蛋白质似乎不共享序列与β-连接蛋白，因为抗β-连接蛋白抗体不识别T，cruzi蛋白，也没有2G 4单克隆抗体识别真正的β-连接蛋白，免疫荧光和免疫金电子显微镜提供的证据表明，这种蛋白质位于细胞内的寄生虫，密切相关的紧身胸衣的四个微管称为膜下微管四重奏。免疫金标记显示，只要鞭毛附着在细胞体上，蛋白质就伴随着鞭毛附着区。有人提出，这些微管相关蛋白的2G 4单克隆抗体识别只存在于锥虫的形式，具有连接复合物之间的鞭毛和细胞体，并可能作为跨膜元件连接膜下微管四重奏与鞭毛在桥粒区域。(C)出版社：Academic Press
Cytoskeletal preparations of Trypanosoma cruzi trypomastigotes and epimastigotes contain a protein recognized by a monoclonal antibody (2G4) which is connected to the flagellar attachment zone of both stages of the parasite. Western blot analysis revealed that the antibody was able to recognize protein bands of molecular masses higher than 700 kDa up to 2500 kDa. These giant proteins do not seem to share sequences with beta-connectin since an anti-beta-connectin antibody did not recognize the T, cruzi proteins nor did the 2G4 monoclonal antibody recognize authentic beta-connectin, Immunofluorescence and immunogold electron microscopy provided evidence that this protein is located inside the cell body of the parasite, closely related to a corset of four microtubules known as subpellicular microtubule quartet. Immunogold labeling shows that the protein accompanies the flagellar attachment zone as long as the flagellum adheres to the cell body. It is proposed that these microtubule-associated proteins recognized by the 2G4 monoclonal antibody exist only in trypanosomatid forms having a junctional complex between the flagellum and the cell body and may act as transmembrane elements connecting the subpellicular microtubular quartet with the flagellum at the desmosome region. (C) 1995 Academic Press, Inc.