Centrosome protein centrin 2/caltractin 1 is part of the xeroderma pigmentosum group C complex that initiates global genome nucleotide excision repair
Centrosome protein centrin 2/caltractin 1 is part of the xeroderma pigmentosum group C complex that initiates global genome nucleotide excision repair
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DOI：
10.1074/jbc.m100855200
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发表时间：
2001-06-01
影响因子：
4.8
通讯作者：
Hanaoka, F
中科院分区：
文献类型：
--
作者：
Araki, M;Masutani, C;Hanaoka, F
核苷酸切除修复（NER）是由着色性干皮病（XP）因子进行的。在切除反应之前，DNA损伤被最初认为含有XP C组责任基因产物（XPC）和Rad 23 B的人同源物（HR 23 B）的复合物识别。在这里，我们表明，中心蛋白2/caltractin 1（CEN 2）也是XPC修复复合物的一个组成部分。我们证明，几乎所有的XPC复合物含有CEN 2，CEN 2直接与XPC相互作用，CEN 2与HR 23 B合作，稳定XPC，刺激XPC NER活性在体外。CEN 2已被证明在中心体复制中起重要作用。因此，这些发现表明XPC-CEN 2相互作用可能反映了细胞分裂和NER的耦合。
Nucleotide excision repair (NER) is carried out by xeroderma pigmentosum (XP) factors. Before the excision reaction, DNA damage is recognized by a complex originally thought to contain the XP group C responsible gene product (XPC) and the human homologue of Rad23 B (HR23B). Here, we show that centrin 2/caltractin 1 (CEN2) is also a component of the XPC repair complex. We demonstrate that nearly all XPC complexes contain CEN2, that CEN2 interacts directly with XPC, and that CEN2, in cooperation with HR23B, stabilizes XPC, which stimulates XPC NER activity in vitro. CEN2 has been shown to play an important role in centrosome duplication. Thus, those findings suggest that the XPC-CEN2 interaction may reflect coupling of cell division and NER.