Pepino mosaic virus isolates and differential symptomatology in tomato
Pepino mosaic virus isolates and differential symptomatology in tomato
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DOI：
10.1111/j.1365-3059.2008.02018.x
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发表时间：
2009-06-01
期刊：
影响因子：
2.7
通讯作者：
Thomma, B. P. H. J.
中科院分区：
文献类型：
--
作者：
Hanssen, I. M.;Paeleman, A.;Thomma, B. P. H. J.
根据2006年在比利时进行的商业番茄生产调查，选择了四种在原产地作物中症状表达不同的Pepino花叶病毒（PepMV）分离株进行温室试验。将所选分离物接种到在四个单独的塑料隧道中生长的番茄植株上。定期评估PepMV症状发展，并进行广泛采样，然后进行ELISA分析、基因分型和测序，以研究整个试验期间PepMV序列中的病毒存在和变异。根据原产作物中的轻度症状表现选择的两个分离株（EU-轻度和CH 2-轻度）在试验中仅引起轻度症状，而选择用于严重症状表现的另外两个分离株（CH 2-侵袭性和EU + CH 2）引起相当严重的症状。CH 2-mild和CH 2-aggressive之间的同源性高达99.4%。这项研究的结果表明，差异的症状表达，至少部分地，可以归因于PepMV分离，这可能与分离株之间的核苷酸水平的微小差异。
Based on a survey conducted in commercial tomato production in Belgium in 2006, four Pepino mosaic virus (PepMV) isolates that differed in symptom expression in the crop of origin were selected for greenhouse trials. The selected isolates were inoculated onto tomato plants grown in four separate plastic tunnels. PepMV symptom development was assessed regularly and extensive sampling followed by ELISA analyses, genotyping and sequencing was performed to study viral presence and variation in PepMV sequences throughout the trial period. Two isolates (EU-mild and CH2-mild) that were selected based on mild symptom expression in the crop of origin caused only mild symptoms in the trial, while two other isolates (CH2-aggressive and EU + CH2) that were selected for severe symptom display, caused considerably more severe symptoms. Sequence homology between CH2-mild and CH2-aggressive was as high as 99.4%. Results of this study show that differential symptom expression can, at least partially, be attributed to the PepMV isolate, which may be related to minor differences at the nucleotide level between isolates.