Clusterin expression level correlates with increased oxidative stress in asthmatics
Clusterin expression level correlates with increased oxidative stress in asthmatics
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DOI：
10.1016/j.anai.2013.12.012
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发表时间：
2014-03-01
影响因子：
5.9
通讯作者：
Cho, You Sook
中科院分区：
文献类型：
--
作者：
Kwon, Hyouk-Soo;Kim, Tae-Bum;Cho, You Sook
背景：氧化应激被认为在哮喘的发病机制中起作用。白藜芦醇是一种敏感的氧化应激细胞生物传感器，具有抗氧化特性。clusterin在哮喘患者中的功能和表达尚未得到充分的研究。目的：探讨哮喘患者clusterin的表达是否受到氧化负荷增加的调节，以及clusterin的表达是否可以用来评估吸入皮质类固醇的反应。血清、诱导痰、用酶联免疫吸附法检测哮喘患者外周血单个核细胞，连接免疫吸附试验和蛋白质印迹法，并与肺功能和过氧化氧还蛋白的表达水平进行比较。在治疗初治患者吸入糖皮质激素use.Results：血清丛生素浓度显着升高，严重哮喘患者，并与肺功能呈负相关。哮喘患者外周血单个核细胞中过氧化氢氧化物的表达显著增加，并与丛生素的表达密切相关。结论：哮喘患者吸入糖皮质激素治疗后，其血清凝集素浓度明显降低，可能是反映哮喘严重程度和氧化应激负荷的生物标志物。此外，clusterin可能有助于迅速评估气道炎症。(C)2014年美国过敏，哮喘和免疫学学院。爱思唯尔公司出版All rights reserved.
Background: Oxidative stress is thought to play a role in the pathogenesis of asthma. Clusterin is a sensitive cellular biosensor of oxidative stress and has antioxidant properties. The function and expression of clusterin in patients with asthma have not been fully investigated.Objective: To investigate whether the expression of clusterin in patients with asthma is regulated by increased oxidative burden and whether clusterin expression could be used to assess the response to inhaled corticosteroids.Methods: Clusterin levels in serum, induced sputum, and peripheral blood mononuclear cells of patients with asthma were measured by enzyme-linked immunosorbent assay and western blotting and compared with pulmonary function and levels of expression of hyperoxidized peroxiredoxins. Serum concentrations of clusterin in treatment-naive patients were compared before and after inhaled corticosteroid use.Results: Serum clusterin concentration was significantly elevated in patients with severe asthma and was inversely correlated with pulmonary function. The expression of hyperoxidized peroxiredoxins was greatly increased in peripheral blood mononuclear cells of patients with asthma and was strongly correlated with clusterin expression. Serum clusterin concentrations in treatment-naive patients with asthma were decreased significantly after initial treatment with inhaled corticosteroids.Conclusion: Clusterin may be a biomarker of asthma severity and the burden of oxidative stress in patients with asthma. Moreover, clusterin may be useful for the prompt assessment of airway inflammation. (C) 2014 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.