siRNAs targeting an intronic transposon in the regulation of natural flowering behavior in Arabidopsis
siRNAs targeting an intronic transposon in the regulation of natural flowering behavior in Arabidopsis
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DOI：
10.1101/gad.1217304
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发表时间：
2004-12-01
影响因子：
10.5
通讯作者：
Chen, XM
中科院分区：
文献类型：
--
作者：
Liu, J;He, YH;Chen, XM
开花基因C(FLC)是开花的中心抑制基因，它的等位基因变异导致了拟南芥不同材料间开花行为的自然差异。在LER的加入中，FLC等位基因的弱性质是由于FLC RNA的低水平，通过一种未知的机制，导致了插入到FLC内含子中的转座元件。在这里，我们表明，转座元件使FLC-LER受到由基因组中同源转座元件产生的短干扰RNA介导的抑制性染色质修饰。我们的研究对转座元件在真核基因表达和进化中的作用具有普遍意义。
Allelic variation in FLOWERING LOCUS C (FLC), a central repressor of flowering, contributes to natural differences in flowering behavior among Arabidopsis accessions. The weak nature of the FLC allele in the Ler accession is due to low levels of FLC RNA resulting, through an unknown mechanism, from a transposable element inserted in an intron of FLC. Here we show that the transposable element renders FLC-Ler subject to repressive chromatin modifications mediated by short interfering RNAs generated from homologous transposable elements in the genome. Our studies have general implications for the role of transposable elements in eukaryotic gene expression and evolution.