Structure of an extracellular cross-reactive polysaccharide from Pseudomonas aeruginosa immunotype 4.
Structure of an extracellular cross-reactive polysaccharide from Pseudomonas aeruginosa immunotype 4.
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铜绿假单胞菌免疫型 4 胞外交叉反应多糖的结构。
DOI：
10.1016/s0021-9258(18)37956-0
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
G. Pier
中科院分区：
文献类型：
--
作者：
N. A. Kocharova;Y. Knirel;A. Shashkov;N. K. Kochetkov;G. Pier
从铜绿假单胞菌免疫型4的培养物上清液中分离包含五糖重复单元的中性小分子量（约6.5kDa）多糖。多糖具有如下的五糖重复单元（式;参见正文），其中Rha是鼠李糖。使用酸水解，溶剂分解与无水氟化氢，甲基化分析，和1H和13 C核磁共振光谱，包括核Overhauser增强实验的结构进行了测定。多糖结合抗体针对七种铜绿假单胞菌Fisher-Devlin免疫型菌株的脂多糖产生。抑制试验表明，这些菌株的上清液中存在血清学相似的多糖。分别在蛋白质免疫印迹和菌落印迹试验中与铜绿假单胞菌免疫型菌株的脂多糖和全细胞结合的多糖的亲和纯化抗体。该多糖似乎含有存在于铜绿假单胞菌脂多糖核心中的抗原决定簇，或者可能代表除了O侧链之外的脂多糖上的另一个次要多糖取代基。
A neutral small molecular mass (approximately 6.5 kDa) polysaccharide comprising a pentasaccharide repeat unit was isolated from culture supernatants of Pseudomonas aeruginosa immunotype 4. The polysaccharide had a pentasaccharide repeating unit as follows (formula; see text) where Rha is rhamnose. The structure was determined using acid hydrolysis, solvolysis with anhydrous hydrogen fluoride, methylation analysis, and 1H and 13C nuclear magnetic resonance spectroscopy including nuclear Overhauser enhancement experiments. The polysaccharide bound antibody raised to the lipopolysaccharide of the seven P. aeruginosa Fisher-Devlin immunotype strains. Inhibition assays demonstrated the presence of a serologically similar polysaccharide in supernatants of these strains. Affinity-purified antibody to the polysaccharide bound to lipopolysaccharide and whole cells of the immunotype strains of P. aeruginosa in a Western immunoblot and colony blot assay, respectively. This polysaccharide seems to contain an antigenic determinant present in the core of the P. aeruginosa lipopolysaccharide or may represent another minor polysaccharide substituent on the lipopolysaccharide in addition to the O side chain.