Effect of donor age on the concentrations of histatins in human parotid and submandibular/sublingual saliva
Effect of donor age on the concentrations of histatins in human parotid and submandibular/sublingual saliva
复制标题
DOI：
10.1016/s0003-9969(00)00047-9
复制
发表时间：
2000-09-01
影响因子：
3
通讯作者：
Dodds, MWJ
中科院分区：
文献类型：
--
作者：
Johnson, DA;Yeh, CK;Dodds, MWJ
组抑素是人类腺唾液中的小蛋白质，具有抗真菌特性。最近的研究表明，口腔念珠菌感染随着年龄的增长而增加，这表明口腔宿主防御能力与年龄有关。本文研究了年龄和生理刺激对唾液组他汀类药物浓度和分泌的影响。采集6名年轻成人在无刺激、机械（咀嚼）和味觉（0.025 M和0.1 M柠檬酸）刺激下的腮腺和下颌下/舌下唾液，通过阳离子聚丙烯酰胺凝胶电泳测定组他汀类药物的浓度和分泌，随后对染色凝胶进行密度扫描。腺体刺激后，腮腺唾液组蛋白浓度（μ g/ml）无显著升高；组蛋白分泌（mu g/min）增加了26倍（p < 0.005；方差分析）。刺激下颌下/舌下唾液导致组蛋白浓度（p < 0.005）和分泌量（p < 0.0005）显著增加。在柠檬酸（0.1 M）刺激的腮腺和下颌下/舌下唾液样本中测定了唾液组他汀类药物的衰老效应，这些样本来自80个个体（分为四个年龄组，男女人数大致相等：35-44岁；45-54岁；55-64岁和65-76岁）。这些病人都没有服用药物或戴假牙。方差分析显示组他汀类药物无性别差异。回归分析显示，腮腺唾液组蛋白浓度（p < 0.002）和分泌量（p < 0.002）以及下颌/舌下唾液组蛋白浓度（p < 0.0001）和分泌量（p < 0.0001）均与年龄相关。两种唾液类型的组蛋白浓度均显著（p < 0.0001）降低，表明与总唾液蛋白相比，唾液组蛋白含量优先降低。这些结果表明，口腔宿主防御系统的唾液组蛋白成分随着年龄的增长而受损。(C) 2000 Elsevier Science Ltd.版权所有。
Histatins are small proteins of human glandular saliva that have antifungal properties. Recent studies show that oral candidal infections increase with age, suggesting an age-associated compromise in oral host defence. Here, the effect of age and of physiological gland stimulation on the concentration and secretion of salivary histatins was investigated. Parotid and submandibular/sublingual salivas were collected from six young adults under unstimulated, mechanical (chewing) and gustatory (0.025 M and 0.1 M citric acid) stimulation, and the concentration and secretion of histatins was measured by cationic polyacrylamide gel electrophoresis with subsequent densitometric scanning of the stained gels. With gland stimulation, parotid saliva showed no significant increase in histatin concentration (mu g/ml); however, histatin secretion (mu g/min) increased up to 26-fold (p < 0.005; ANOVA). Stimulation of submandibular/sublingual saliva resulted in significant increases in both histatin concentration (p < 0.005) and secretion (p < 0.0005). Ageing effects on salivary histatins were determined in citric acid (0.1 M)-stimulated parotid and submandibular/sublingual saliva samples collected from 80 individuals (divided into four age groups having approximately equal numbers of males and females: 35-44 years; 45-54 years; 55-64 years and 65-76 years). None of the patients was taking medications or wore dentures. ANOVA showed no sex differences in histatins. Regression analysis showed significant age-associated decreases for parotid saliva histatin concentration (p < 0.002) and secretion (p < 0.002) as well as for submandibular/sublingual saliva histatin concentration (p < 0.0001) and secretion (p < 0.0001). Both saliva types showed significant (p < 0.0001) decreases in the histatin concentration per mg of total protein, suggesting a preferential decrease in salivary histatins compared to total salivary protein. These results suggest that the salivary histatin component of the oral host defence system is compromised with increasing age. (C) 2000 Elsevier Science Ltd. All rights reserved.