Genome-wide identification and expression analysis of rice NLR genes responsive to the infections of Xanthomonas oryzae pv. oryzae and Magnaporthe oryzae
Genome-wide identification and expression analysis of rice NLR genes responsive to the infections of Xanthomonas oryzae pv. oryzae and Magnaporthe oryzae
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DOI：
10.1016/j.pmpp.2020.101488
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发表时间：
2020-08-01
影响因子：
2.7
通讯作者：
Zou, Lifang
中科院分区：
文献类型：
--
作者：
Ding, Li;Xu, Xiameng;Zou, Lifang
核苷酸结合位点富亮氨酸重复序列（NLR）基因在水稻抗病中起关键作用。然而，水稻NLR基因在病原菌入侵过程中的转录活性尚不清楚。为了揭示它们的活性，我们鉴定了430个具有NBS和LRR结构域的水稻NLR基因，其中包括192个CNL和238个XNL（不含CC基序）成员。我们对69份感染水稻黄单胞菌的水稻微阵列样品进行了个体和综合分析。oryzae （Xoo）和Magnaporthe oryzae （more）。在水稻NLR基因中，397个基因低/中表达，10个基因高表达。在至少一个样本中发现了总共400个NLR基因的差异表达。此外，鉴定出46个水稻NLR基因对两种病原菌的差异表达，其中38个基因可以通过RNA-seq数据进行验证。6个顺式调控元件（MYC、STRE、MYB、ABRE、G-box和AS-1）在水稻NLR基因启动子区域频繁出现。选择10个NLR基因进行qPCR检测，其中7个NLR基因验证了芯片和RNA-seq数据的有效性。本研究结果有助于揭示NLR基因在水稻抗Xoo和Mor中的表达和调控。
Nucleotide-binding site, leucine-rich repeat (NLR) genes play a critical role in rice disease resistance. However, the transcriptional activities of rice NLR genes during pathogen invasions are still unclear. To uncover their activities, we identified 430 regular rice NLR genes with both NBS and LRR domains, consisting of 192 CNL and 238 XNL (without a CC motif) members. We performed individual and integrative analyses based on 69 samples from rice microarray after the infections of Xanthomonas oryzae pv. oryzae (Xoo) and Magnaporthe oryzae (Mor). Among the rice NLR genes, 397 were found to be expressed at low/medium level, while 10 NLR genes were observed to show high expression levels. A total of 400 NLR genes were discovered to be differentially expressed in at least one sample. Furthermore, 46 rice NLR genes were identified to be differentially expressed in response to the two pathogens and 38 of them could be validated using RNA-seq data. Six cis-regulatory elements (MYC, STRE, MYB, ABRE, G-box, and AS-1) were observed to occur frequently in the promoter regions of rice NLR genes. Ten NLR genes were selected for qPCR detection, and seven of the NLR genes verified the validity of the microarray and RNA-seq data. Our results are helpful in revealing expressions and regulations of NLR genes in rice resistance to Xoo and Mor.