Analysis of intact rat lens metabolites by P-31 NMR spectroscopy.
Analysis of intact rat lens metabolites by P-31 NMR spectroscopy.
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通过 P-31 NMR 光谱分析完整的大鼠晶状体代谢物。
DOI：
10.3109/02713688109019974
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发表时间：
1981
影响因子：
2
通讯作者：
T. Glonek
中科院分区：
文献类型：
--
作者：
S. J. Kopp;J. Greiner;T. Glonek
利用磷-31核磁共振（P-31 NMR）技术对大鼠晶状体进行了评价，认为其可作为研究人类晶状体代谢过程的潜在动物模型。完整的大鼠晶状体和大鼠晶状体PCA提取物的P-31 NMR谱在中间代谢的含磷代谢物的组织水平上提供了相当的定性和定量结果。本研究还建立了用P-31核磁共振检测完整大鼠晶状体代谢的可行性。与已报道的人和兔晶状体代谢物水平进行比较，发现存在显著差异；因此，从大鼠晶状体研究中获得的代谢信息应谨慎外推到人类身上。
Using phosphorus-31 nuclear magnetic resonance (P-31 NMR) spectroscopy the rat crystalline lens was evaluated as a potential animal model for the study of lens metabolic processes relevant to the human. The P-31 NMR spectra of intact rat lenses and rat lens PCA extracts provided comparable qualitative and quantitative results concerning the tissue levels of the phosphorus-containing metabolites of intermediate metabolism. The study also establishes the feasibility of examining intact rat lens metabolism by P-31 NMR. Comparisons with reported human and rabbit lens metabolite levels revealed significant differences; consequently, metabolic information derived from rat lens studies should be extrapolated to the human with caution.