Pharmacology of mammalian olfactory receptors.
Pharmacology of mammalian olfactory receptors.
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哺乳动物嗅觉受体的药理学。
DOI：
10.1007/978-1-62703-377-0_15
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Araneda,RicardoC
中科院分区：
文献类型：
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作者：
Smith,RichardS;Peterlin,Zita;Araneda,RicardoC
哺乳动物物种已经进化出大量且多样的气味受体（OR）。这些蛋白质组成了已知的最大的G蛋白偶联受体（GPCR）家族，在啮齿动物中有大约1,000种不同的受体。从嗅觉编码的角度来看，如此大量的化学感受受体的可用性提出了几个迷人的问题;此外，如此大的剧目提供了一个有吸引力的生物模型来研究配体-受体相互作用。然而，这些受体在异源系统中的有限功能表达极大地阻碍了将它们去乙酰化的尝试。我们采用了一种成功的方法，结合电生理和成像技术来分析单个感觉神经元的反应概况。我们的方法使我们能够表征天然醛受体群体的“气味空间”和基因工程受体OR-I7的分子范围。
Mammalian species have evolved a large and diverse number of odorant receptors (ORs). These proteins comprise the largest family of G-protein-coupled receptors (GPCRs) known, amounting to ∼1,000 different receptors in the rodent. From the perspective of olfactory coding, the availability of such a vast number of chemosensory receptors poses several fascinating questions; in addition, such a large repertoire provides an attractive biological model to study ligand–receptor interactions. The limited functional expression of these receptors in heterologous systems, however, has greatly hampered attempts to deorphanize them. We have employed a successful approach that combines electrophysiological and imaging techniques to analyze the response profiles of single sensory neurons. Our approach has enabled us to characterize the “odor space” of a population of native aldehyde receptors and the molecular range of a genetically engineered receptor, OR-I7.