[Comparative study on lipogenic and lipolytic gene expression in intramuscular fat tissue between growing Erhualian and large white pigs].
[Comparative study on lipogenic and lipolytic gene expression in intramuscular fat tissue between growing Erhualian and large white pigs].
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发表时间：
2004-11
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通讯作者：
Q. Gao;Jun Li;Hong-lin Liu;Lin-Yun Wang;Yin-xue Xu
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作者：
Q. Gao;Jun Li;Hong-lin Liu;Lin-Yun Wang;Yin-xue Xu
为了探讨二花脸猪和大白猪肌内脂肪沉积差异的机理，采用单管相对定量RT-PCR方法，以18S内标为对照，研究了生脂(ACX、LPL、ME)和脂解(HSL)基因表达的发育规律。选用16头大白猪和20头二花脸公猪，分别按正常营养标准饲养。随机抽取大白猪15 kg、40 kg、60 kg、90 kg和二花脸猪18 kg、40 kg、60 kg、80 kg、90 kg屠宰，每个品种4头。分别取冈上肌和半膜肌进行总RNA提取，背最长肌采用乙醚提取法进行肌内脂肪分析。结果表明：(1)二花脸公猪与大白猪在生长早期(40 kg以前)的IMF特性相似(P>；0.05)，此后，二花脸公猪的IMF水平在60 kg和90 kg时分别急剧上升到4%和5%以上，而大白猪则稳定地保持在2%左右(P&t；0.05)；(3)二花脸猪在20~60 kg体重范围内，LPL和ME基因的表达趋势与IMF的发育趋势一致。结果表明，二花脸猪在20~60 kg体重范围内发育可能对其沉积起重要作用，ME和LPL基因的表达可能有助于二花脸猪IMF的快速沉积。
To investigate the mechanism of difference in intramuscular fat deposition between Erhualian and Large White pigs,single tube relative-quantitative RT-PCR method was used to investigate the development patterns of lipogenic (ACX, LPL, ME) and lipolytic (HSL) gene expression with 18S internal standard control. Sixteen Large White boars and twenty Erhualian boars were selected and raised according to normal nutrition standard respectively. The animals were selected randomly and slaughtered at 15 kg, 40 kg, 60 kg and 90 kg for Large White pigs and at 18 kg, 40 kg, 60 kg, 80 kg and 90 kg for Erhualian pigs respectively; with four animals of each breed at each time. The supraspinatus and semimembranosus muscles were removed for total RNA extraction and longisimus dorsi muscle for intramuscular fat (IMF) analysis using ether extract method. The results showed: (1) The property of IMF between Erhualian and Large White boars was similar during early growing period (before 40 kg) (P > 0.05) ,thereafter, IMF level of Erhualian boars increased dramatically to 4% at 60 kg and over 5% at 90 kg while Large White boars kept steadily at about 2% (P < 0.05); (2) The pattern of lipogenic and lypolytic gene expression was similar between semimembranosus and supraspinatus muscle in each breed; (3) The tendency for LPL and ME mRNA expression coincided with that of IMF development among 20 approximately 60 kg in Erhualian pigs. The results suggest that the development of IMF between 20 kg and 60 kg in Erhualian pigs may play a meaningful role in deposition of IMF,and that the expression of ME and LPL mRNA may contribute to fast sediment of IMF in Erhualian pigs.