Molecular characterisation of a hypervariable region downstream of the human alpha‐globin gene cluster.
Molecular characterisation of a hypervariable region downstream of the human alpha‐globin gene cluster.
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人类α珠蛋白基因簇下游高变区的分子特征。
DOI：
10.1002/j.1460-2075.1986.tb04437.x
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
D. Higgs
中科院分区：
文献类型：
--
作者：
A. Jarman;R. Nicholls;D. Weatherall;J. Clegg;D. Higgs
我们已经确定了位于人类α-珠蛋白基因复合体下游8kb的DNA的一个不寻常的、高度多态的区域。这个高变区(α-珠蛋白3‘hvr)由一组17bp的串联重复序列组成，其数量在不同的等位基因之间有很大的差异(70-450)。17个碱基重复序列在等位基因内部和等位基因之间高度保守。此外，该序列鉴定了一个核心寡核苷酸[5‘-GNGGGG(N)ACAG-3’]，它是三个先前特征的高变区共有的。在严格程度降低的情况下，对3‘HVR的探针检测到了一组新的多等位基因座，这将在人类遗传学研究中具有价值。
We have characterised an unusual, highly polymorphic region of DNA located 8‐kb downstream of the human alpha‐globin gene complex. This hypervariable region (alpha‐globin 3′ HVR) is composed of an array of 17‐bp tandem repeats, the number of which differs considerably (70‐450) from one allele to another. The sequence of the 17‐bp repeats is highly conserved within and between alleles. Furthermore, this sequence identifies a core oligonucleotide [5′‐GNGGGG(N)ACAG‐3′] that is common to three previously characterised hypervariable regions. At reduced stringency, a probe to the 3′ HVR detects a new family of multiallelic loci that will be of value in the study of human genetics.