An Artefact in Quantitative Immunoelectrophoresis of Spectrin Caused by Proteolytic Activity in Antibody Preparations
An Artefact in Quantitative Immunoelectrophoresis of Spectrin Caused by Proteolytic Activity in Antibody Preparations
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抗体制剂中蛋白水解活性引起的血影蛋白定量免疫电泳中的伪影
DOI：
10.1111/j.1365-3083.1975.tb03812.x
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发表时间：
1975
影响因子：
3.7
通讯作者：
T. Bøg‐Hansen
中科院分区：
文献类型：
--
作者：
O. Bjerrum;J. Ramlau;I. Clemmesen;A. Ingild;T. Bøg‐Hansen
由于纤溶酶的存在，许多抗体制剂表现出蛋白水解活性。在 pH 8.6 的交叉免疫电泳中，这种酶可以在含抗体凝胶的电泳过程中降解某些蛋白质，从而产生形状一致的额外沉淀弧形式的伪影。研究了血影蛋白（人红细胞膜的主要蛋白质）的降解行为。通过添加蛋白酶抑制剂（例如蛋白酶抑制剂）可以完全消除这种假象。抑肽酶和大豆胰蛋白酶抑制剂，用于抗体制剂。
Many antibody preparations exhibit proteolytic activity due to the presence of plasmin. In crossed immunoelectrophoresis at pH 8.6 this enzyme can degrade certain proteins during electrophoresis in the antibody‐containing gel, resulting in artefacts in the form of extra precipitation arcs of congruent shape. The degradation behaviour of spectrin, a major protein of human erythrocyte membranes, was investigated. The artefact could be completely abolished by the addition of protease inhibitors, e.g. aprotinin and soya bean trypsin inhibitor, to the antibody preparations.