PAS-histidine kinases PHK1 and PHK2 exert oxygen-dependent dual and opposite effects on gametophore formation in the moss Physcomitrella patens.
PAS-histidine kinases PHK1 and PHK2 exert oxygen-dependent dual and opposite effects on gametophore formation in the moss Physcomitrella patens.
复制标题
PAS-组氨酸激酶 PHK1 和 PHK2 对小立碗藓 (Physcomitrella patens) 配子体形成发挥氧依赖性双重且相反的作用。
DOI：
10.1016/j.bbrc.2018.08.056
复制
发表时间：
2018
影响因子：
3.1
通讯作者：
Yuichi Fujita and Setsuyuki Aoki.
中科院分区：
文献类型：
--
作者：
Masashi Ryo;Takafumi Yamashino,Hisanori Yamakawa;Yuichi Fujita and Setsuyuki Aoki.
双组分系统是一种基于磷酸盐在组分蛋白之间转移的多功能信号传导机制，在陆地植物进化的适应和多样化过程中发挥了重要作用。我们以前证明，两个Per-Arnt-Sim（PAS）-组氨酸激酶，PHK 1和PHK 2，抑制配子体形成的mossPhyscomitrella patensunder有氧条件下，在真核生物中，其同系物的存在仅限于早期分歧的链霉菌谱系。我们在此评估PHK是否在氧信号传导中发挥作用。当浸没在水中时，PHK 1和PHK 2的双破坏系在光暗循环或连续光照下形成的配子体比野生型系（WT）少，这表明PHK在水生环境下促进配子体的形成，与有氧条件相反。同样，在人工低氧条件下，双分裂系形成的配子体比WT少。这些结果表明，PHKs发挥双重和相反的影响配子体的形成取决于氧状态。这项研究增加了重要的洞察功能的多样性和进化意义的两个组成部分系统在陆地植物。
Two-component systems, versatile signaling mechanisms based on phosphate transfer between component proteins, must have played important roles in adaptation and diversification processes in land plant evolution. We previously demonstrated that two Per-Arnt-Sim (PAS)-histidine kinases, PHK1 and PHK2, repress gametophore formation in the mossPhyscomitrella patensunder aerobic conditions, and that, in eukaryotes, the presence of their homologs is restricted to early-diverging streptophyte linages. We assessed here whether or not PHKs play a role in oxygen signaling. When submerged under water, the double disruption line forPHK1andPHK2formed fewer gametophores than the wild-type line (WT) both under light-dark cycles or continuous light, indicating that PHKs promote gametophore formation under an aquatic environment, in contrast to aerobic conditions. Similarly, in an artificial low-oxygen condition, the double disruption line formed fewer gametophores than WT. These results indicate that PHKs exert dual and opposite effects on gametophore formation depending on oxygen status. This study adds important insight into functional versatility and evolutionary significance of two-component systems in land plants.