DEVELOPMENT OF THE B-CELL AND T-CELL COMPARTMENTS IN PORCINE LYMPHOID ORGANS FROM BIRTH TO ADULT LIFE - AN IMMUNOHISTOLOGICAL APPROACH
DEVELOPMENT OF THE B-CELL AND T-CELL COMPARTMENTS IN PORCINE LYMPHOID ORGANS FROM BIRTH TO ADULT LIFE - AN IMMUNOHISTOLOGICAL APPROACH
复制标题
DOI：
10.1016/0165-2427(92)90182-p
复制
发表时间：
1992-08-01
影响因子：
1.8
通讯作者：
MOONENLEUSEN, HWM
中科院分区：
文献类型：
--
作者：
BIANCHI, ATJ;ZWART, RJ;MOONENLEUSEN, HWM
使用免疫组织学技术，我们研究了随着时间的推移，B-和T-细胞室在特定病原体的猪淋巴器官的发展。在不同的时间点收集组织样品，从猪出生前2天开始，直到猪10个月大。从脾脏、胸腺、外周淋巴结、肠系膜淋巴结、十二指肠、空肠、回肠、空肠派伊尔集合淋巴结和回肠派伊尔集合淋巴结采集样品。使用对B细胞和T细胞具有特异性的单克隆抗体来鉴定以下细胞的定位位置：IgM-B细胞（表面免疫球蛋白阳性细胞）、含IgM、IgG和IgA的细胞（细胞质免疫球蛋白阳性细胞）以及CD 2、CD 4和CD 8阳性细胞。分析了每个器官中B和T细胞亚群的发育。出生前两天，大多数器官含有大量的IgM-B细胞。脾脏、淋巴结、派伊尔集合淋巴结，尤其是胸腺，含有一些含免疫球蛋白的细胞（Ig-CC）;这一发现表明猪在出生前就有分泌免疫球蛋白的细胞。刚出生后，Ig-CC的发病率在大多数器官中增加;首先IgM-CC增加，然后IgG或IgA-CC增加，这取决于器官。在脾脏和小肠固有层中清楚地观察到T细胞发育，与其他器官相反，其中含有各种T细胞亚群的T细胞隔室在出生前发育良好。比较CD 4+和CD 8+细胞的发生率表明，与其他物种的CD 4：CD 8比率相比，脾脏、淋巴结、派伊尔集合淋巴结和小肠中这些细胞的CD 4：CD 8比率较低，尤其是在成年猪中。断奶对淋巴器官中B细胞和T细胞的发生率影响不大。这项研究是第一个免疫组织学调查，以描述的发展主要B-和T-细胞亚群在各种淋巴器官的猪，它应该是有用的，为未来的免疫病理学和比较免疫学研究在猪。
Using immunohistological techniques, we studied the development over time of B- and T-cell compartments in the lymphoid organs of specific-pathogen-free pigs. Tissue samples were collected at various time-points, starting 2 days before the pigs were born until the pigs were 10 months old. The samples were collected from the spleen, thymus, peripheral lymph node, mesenteric lymph node, duodenum, jejunum, ileum, jejunal Peyer's patch and ileal Peyer's patch. Monoclonal antibodies specific to B- and T-cells were used to identify where the following cells were localized: IgM-B cells (cells positive to surface immunoglobulin), IgM-, IgG- and IgA-containing cells (cells positive to cytoplasmic immunoglobulin), and CD2-, CD4- and CD8-positive cells. The development of the B- and T-cell subpopulations in each organ was analysed. Two days before birth, most organs contained quantities of IgM-B cells. The spleen, lymph nodes, Peyer's patches and, notably, the thymus, contained some immunoglobulin-containing cells (Ig-CC); this finding indicates that pigs have cells that secrete immunoglobulins before birth. Just after birth, the incidence of Ig-CC increased in most organs; first IgM-CC increased, then either IgG- or IgA-CC increased, depending on the organ. T-cell development was observed clearly in spleen and in the lamina propria of the small intestine, in contrast to other organs, in which the T-cell compartments containing various T-cell subpopulations were well developed before birth. Comparison of the incidence of CD4+ and CD8+ cells showed that the CD4:CD8 ratio of these cells in the spleen, lymph nodes, Peyer's patches and small intestine is low, especially in adult pigs, compared with the CD4:CD8 ratio in other species. Weaning had little influence on the incidence of B- and T-cells in lymphoid organs. This study is the first immunohistological survey to describe the development of the major B- and T-cell subpopulations in various lymphoid organs of pigs, and it should be useful for future immunopathological and comparative immunological studies in pigs.