Severity of diabetic microvascular complications is associated with a low soluble RAGE level
Severity of diabetic microvascular complications is associated with a low soluble RAGE level
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DOI：
10.1016/j.diabet.2008.04.003
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发表时间：
2008-09-01
影响因子：
7.2
通讯作者：
Wautier, J. -L.
中科院分区：
文献类型：
--
作者：
Grossin, N.;Wautier, M. -P.;Wautier, J. -L.
目标。晚期糖基化终产物受体（RAGE）与糖尿病微血管并发症有关，但多项证据表明，RAGE的可溶性异构体（sRAGE）可防止age介导的血管损伤。典型的AGE N ε -羧甲基lsin （CML）与糖尿病微血管并发症有关。在本研究中，我们测量了有微血管并发症和无微血管并发症的糖尿病患者血液中sRAGE和cml蛋白的水平。研究招募了30名2型糖尿病患者，其中20名没有微血管并发症，10名同时患有视网膜和肾脏并发症。ELISA法测定血清中sRAGE，竞争ELISA法测定CML。-对照组和无微血管并发症的糖尿病患者的sRAGE水平相似。与无并发症的糖尿病患者相比，有并发症患者的平均sRAGE血药浓度显著降低（1068 +/- 231 pg/mL） （P=0.028）。cml蛋白在所有糖尿病患者中均升高，但在有微血管并发症的患者中升高的程度更高。-在发生严重糖尿病并发症的糖尿病患者中，低sRAGE与高cml蛋白水平的关联支持了sRAGE保护血管免受age介导的糖尿病微血管损伤的假设。(c) 2008 Elsevier Masson SAS。版权所有。
Aims. - The receptor for advanced glycation end-products (RAGE) has been implicated in diabetic microvascular complications, but several lines of evidence suggest that the soluble isoform of RAGE (sRAGE) may protect against AGE-mediated vessel damage. The characterized AGE N epsilon-carboxymethyllsine (CML) is associated with diabetic microvascular complications. In the present study, we measured blood levels of sRAGE and CML-protein in diabetic patients with and without microvascular complications.Methods. - Thirty patients with type-2 diabetes were recruited into the study, comprising 20 who had no microvascular complications, and 10 who had both retinal and renal complications. sRAGE was measured in serum by ELISA, and CML by competitive ELISA.Results. - sRAGE blood lelvels were similar in both the controls and diabettic patients without microvascular complications. In patients with complications, the mean sRAGE blood level was significantly decreased (1068 +/- 231 pg/mL) compared with diabetic patients without complications (P=0.028). CML-protein was increased in all diabetic patients, but to a higher extent in those who had microvascular complications.Conclusion. - The association of low sRAGE with high CML-protein levels in diabetic patients who developed severe diabetic complications supported the hypothesis that sRAGE protects vessels against AGE-mediated diabetic microvascular damage. (c) 2008 Elsevier Masson SAS. All rights reserved.