Detection of species-specific antibody response of humans and mice bitten by sand flies
Detection of species-specific antibody response of humans and mice bitten by sand flies
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DOI：
10.1017/s003118200400681x
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发表时间：
2005-05-01
期刊：
影响因子：
2.4
通讯作者：
Volf, P
中科院分区：
文献类型：
--
作者：
Rohousova, I;Ozensoy, S;Volf, P
白蛉唾液在利什曼原虫传播中发挥着重要作用。我们表征了 3 种白蛉的宿主抗体对唾液的反应。在实验性咬伤小鼠的血清以及居住在土耳其桑尼乌法热带利什曼原虫流行区的个体的血清中观察到特异性 IgG。桑尼乌法居民的血清显示出针对该地区两种最丰富的白蛉物种——白蛉（Phlebotomus sergenti）和帕帕塔西（P. papatasi）唾液的高 IgG 水平，但与新世界白蛉（Lutzomyia longipalpis）的唾液不发生反应。活动性Le患者。热带病灶具有显着较高的抗P。 sergenti IgG 水平高于同一地点的健康个体，同时抗 P.两组的 papatasi IgG 水平相等。 P. papatasi 和 P. sergenti 唾液中的主要蛋白质条带与人类和小鼠血清发生反应；然而，在 P. papatasi 中，小鼠 IgG 优先识别 42 kDa 蛋白带，而人 IgG 与 30 kDa 蛋白带强烈反应。我们的数据表明，对白蛉唾液的抗体反应可用于监测人类和其他宿主对白蛉的暴露情况，并可用作利什曼原虫在流行地区传播风险的标志。
Sand fly saliva plays an important role in Leishmania transmission. We characterized the host antibody response to saliva from 3 sand fly species. Specific IgG was observed in sera from experimentally bitten mice as well as in sera from individuals living in the endemic area of Leishmania tropica in Sanliurfa, Turkey. Sera of Sanliurfa inhabitants showed high IgG levels against saliva of Phlebotomus sergenti and P. papatasi, the 2 most abundant sand fly species in this area, but did not react with saliva of the New World sand fly, Lutzomyia longipalpis. Patients with active Le. tropica lesions possessed significantly higher anti-P. sergenti IgG levels than the healthy individuals from the same place while anti-P. papatasi IgG levels were equal in both groups. Major protein bands in P. papatasi and P. sergenti saliva reacted with both, human and mice sera; in P. papatasi, however, mouse IgG recognized preferentially the 42 kDa protein band while the human IgG reacted strongly with the 30 kDa band. Our data suggest that the antibody response to sand fly saliva could be used for monitoring the exposure of humans and other hosts to sand flies and might be used as a marker of risks for Leishmania transmission in endemic areas.