Extra-embryonic human Wharton's jelly stem cells do not induce tumorigenesis, unlike human embryonic stem cells
Extra-embryonic human Wharton's jelly stem cells do not induce tumorigenesis, unlike human embryonic stem cells
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DOI：
10.1016/j.rbmo.2011.10.007
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发表时间：
2012-02-01
影响因子：
4
通讯作者：
Bongso, Ariff
中科院分区：
文献类型：
--
作者：
Gauthaman, Kalamegam;Fong, Chui-Yee;Bongso, Ariff
肿瘤发生是人胚胎干细胞（ESC）和人诱导多能干细胞（IPSC）来源的组织移植治疗的主要障碍。这促使人们寻找ESC的其他来源。本研究从胚胎外的人脐带华顿氏胶（WJSC）中分离并鉴定了干细胞。这些细胞是无争议的，可大量获得，增殖，多能和低免疫原性。然而，它们的致瘤潜力尚未得到适当的解决。使用免疫缺陷小鼠模型将它们的肿瘤产生能力与人ESC进行比较。将未标记的人ESC +基质胶（2 × 10（6）细胞/位点）、标记的人WJSC（红色荧光蛋白; 5 × 10（6）细胞/位点）和未标记的人WJSC +基质胶（5 × 10（6）细胞/位点）通过三种途径（s.c.，I.M.和i. p.）。接受人ESC +基质胶的动物在6周内（s.c. 85%; i.m. 75%; i.p.100%），其含有外胚层、中胚层和内胚层的组织。接受人WJSC的动物在维持较长时间（20周）时，注射部位未发生肿瘤或炎症反应。与增加促炎细胞因子的人ESC相比，人WJSC产生抗炎细胞因子的增加。人WJSC是低免疫原性和非致瘤性的，具有安全的基于细胞的疗法的潜力。(C)2011年，爱思唯尔有限公司出版的生殖保健有限公司。版权所有。
Tumorigenesis is the major obstacle of tissues derived from human embryonic stem cells (ESC) and human induced pluripotent stem cell (IPSC) for transplantation therapy. This prompted a search for other sources of ESC. This study isolated and characterized stem cells from the extra-embryonic human umbilical cord Wharton's jelly (WJSC). These cells are non-controversial, available in abundance, proliferative, multipotent and hypoimmunogenic. However, their tumorigenic potential has not been properly addressed. Their tumour-producing capabilities were compared with human ESC using the immunodeficient mouse model. Unlabelled human ESC + matrigel (2 x 10(6) cells/site), labelled human WJSC (red fluorescent protein; 5 x 10(6) cells/site) and unlabelled human WJSC + matrigel (5 x 10(6) cells/site) were injected via three routes (s.c., i.m. and i.p.). Animals that received human ESC + matrigel developed teratomas in 6 weeks (s.c. 85%; i.m. 75%; i.p. 100%) that contained tissues of ectoderm, mesoderm and endoderm. No animal that received human WJSC developed tumours or inflammatory reactions at the injection sites when maintained for a prolonged period (20 weeks). Human WJSC produced increases in anti-inflammatory cytokines in contrast to human ESC, which increased pro-inflammatory cytokines. Human WJSC, being hypoimmunogenic and non-tumorigenic, have the potential for safe cell-based therapies. (C) 2011, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.