Virulence Factors of Geminivirus Interact with MYC2 to Subvert Plant Resistance and Promote Vector Performance
Virulence Factors of Geminivirus Interact with MYC2 to Subvert Plant Resistance and Promote Vector Performance
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DOI：
10.1105/tpc.114.133181
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发表时间：
2014-12-01
期刊：
影响因子：
11.6
通讯作者：
Ye, Jian
中科院分区：
文献类型：
--
作者：
Li, Ran;Weldegergis, Berhane T.;Ye, Jian
病原体可使受感染的植物促进其传播媒介的性能，从而加速病原体的传播。病原体通过它们共同的宿主植物对其媒介的这种积极影响被称为间接互利共生。例如，萜烯生物合成在被菜豆花叶病毒感染的植物中被抑制，导致植物抗性降低和传播这些病毒的烟粉虱（Bemisia tabaci）的性能增强。虽然菜豆花叶病毒-粉虱共生关系已经被人们所了解，但其潜在的机制仍然是难以捉摸的。在这里，我们确定了中国番茄黄化曲叶病毒的β C1，一种单分裂的菜豆花叶病毒，作为抑制植物萜烯生物合成的病毒遗传因子。bC 1直接与碱性螺旋-环-螺旋转录因子MYC 2相互作用，以损害MYC 2调节的萜烯合酶基因的激活，从而降低粉虱抗性。MYC 2与双组分菜豆花叶病毒蛋白BV 1相关联，表明MYC 2是菜豆花叶病毒的进化上保守的靶点，用于抑制基于terterm的抗性和促进载体性能。我们的研究结果描述了这种病毒病原体如何调节宿主植物的代谢，以建立互利共生与其昆虫载体。
A pathogen may cause infected plants to promote the performance of its transmitting vector, which accelerates the spread of the pathogen. This positive effect of a pathogen on its vector via their shared host plant is termed indirect mutualism. For example, terpene biosynthesis is suppressed in begomovirus-infected plants, leading to reduced plant resistance and enhanced performance of the whiteflies (Bemisia tabaci) that transmit these viruses. Although begomovirus-whitefly mutualism has been known, the underlying mechanism is still elusive. Here, we identified beta C1 of Tomato yellow leaf curl China virus, a monopartite begomovirus, as the viral genetic factor that suppresses plant terpene biosynthesis. bC1 directly interacts with the basic helix-loop-helix transcription factor MYC2 to compromise the activation of MYC2-regulated terpene synthase genes, thereby reducing whitefly resistance. MYC2 associates with the bipartite begomoviral protein BV1, suggesting that MYC2 is an evolutionarily conserved target of begomoviruses for the suppression of terpene-based resistance and the promotion of vector performance. Our findings describe how this viral pathogen regulates host plant metabolism to establish mutualism with its insect vector.