Ocular-side lateralization of adult-type chromatophore precursors: development of pigment asymmetry in metamorphosing flounder larvae.
Ocular-side lateralization of adult-type chromatophore precursors: development of pigment asymmetry in metamorphosing flounder larvae.
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DOI：
10.1002/jez.b.22491
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发表时间：
2013-05
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影响因子：
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通讯作者：
Youhei Washio;M. Aritaki;Y. Fujinami;D. Shimizu;Hayato Yokoi;Tohru Suzuki
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作者：
Youhei Washio;M. Aritaki;Y. Fujinami;D. Shimizu;Hayato Yokoi;Tohru Suzuki
牙鲆成虫型色素细胞在眼侧皮肤变态分化，形成不对称的色素沉着。在幼体幼虫和变态之前，在变态过程中迁移到眼侧的成体型黑色素细胞作为潜在的前体存在于背鳍的底部。然而，这些前体细胞的迁移途径以及眼侧侧化和不对称色素沉着形成的机制尚不清楚。为了进一步研究这种迁移和偏侧化，我们使用gch2探针（黑色素母细胞和黄原细胞（成虫型色素体的前体）的标记物）原位杂交来检测色素体前体在蜕变幼虫中的分布。肌隔和皮肤中均有gch2阳性前体。这一发现表明，这些前体通过肌隔从鳍的背部迁移到皮肤。此外，盲侧肌隔中的gch2阳性细胞比眼侧皮肤和眼侧肌隔中的gch2阳性细胞少得多，这一发现表明，前体细胞向盲侧肌隔的迁移受到抑制，或者前体细胞从盲侧肌隔中被消除。因此，我们认为导致比目鱼不对称色素沉着的信号不仅存在于皮肤中，而且存在于更大范围内，存在于躯干外侧的多个组织中。
The adult-type chromatophores of flounder differentiate at metamorphosis in the skin of ocular side to establish asymmetric pigmentation. In young larva and before metamorphosis, adult-type melanophores that migrate to the ocular side during metamorphosis reside at the base of the dorsal fin as latent precursors. However, the migration route taken by these precursor cells and the mechanisms by which lateralization and asymmetric pigmentation develop on the ocular side are unknown. To further investigate this migration and lateralization, we used in situ hybridization with gch2 probe, a marker for melanoblasts and xanthoblasts (precursors of adult type chromatophores), to examine the distribution of chromatophore precursors in metamorphosing larvae. The gch2-positive precursors were present in the myoseptum as well as in the skin. This finding indicated that these precursors migrated from the dorsal part of the fin to the skin via the myoseptum. Additionally, there were much fewer gch2-positive cells in the myoseptum of the blind side than in the skin and myoseptum of the ocular side, and this finding indicated either that migration of the precursor cells into the myoseptum of blind side was inhibited or that the precursors were eliminated from the myoseptum of the blind side. Therefore, we propose that the signals responsible for development of asymmetric pigmentation in flounder reside not only in the skin but on a larger scale and in multiple tissues throughout the lateral half of the trunk.