Highly purified mutant E112K of cholera toxin elicits protective lung mucosal immunity to diphtheria toxin.
Highly purified mutant E112K of cholera toxin elicits protective lung mucosal immunity to diphtheria toxin.
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高度纯化的霍乱毒素突变体 E112K 可引发对白喉毒素的保护性肺粘膜免疫。
DOI：
10.1016/s0264-410x(01)00412-1
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发表时间：
2001
期刊：
影响因子：
5.5
通讯作者：
McGhee,JR
中科院分区：
文献类型：
--
作者：
Ohmura,M;Yamamoto,M;Kiyono,H;Fujihashi,K;Takeda,Y;McGhee,JR
We demonstrated that the mutant of cholera toxin (mCT) E112K which was LPS-free supported the induction of protective immunity in mucosal (e.g. lung lavage) and systemic (e.g. serum) compartments when given nasally with vaccine-grade diphtheria toxoid (DT) to mice. Significant DT-specific mucosal IgA antibody (Ab) and serum IgG, IgA and IgM Ab responses were induced when LPS-depleted mCT E112K or native CT (nCT) was co-administered nasally with DT. The analysis of DT-specific Ab-forming cell (AFC) responses supported the Ab titers and significant numbers of DT-specific IgA AFC were present in the lungs, nasal passages and submandibular glands. Furthermore, DT-specific IgG AFC in cervical lymph nodes (CLN) and the spleen were induced in mice administered with DT nasally with either mCT or nCT. The analysis of antigen-specific T cell responses revealed that increased DT-specific CD4+T cell proliferative and Th2-type cytokine responses were induced in mice nasally-immunized with DT and the LPS-free form of mCT. The neutralization of diphtheria toxin by Abs showed that DT-specific IgG Ab responses in serum and lung lavages of mice immunized with DT and mCT were protective. Furthermore, it was shown that an IgA-enriched fraction of lung lavages possessed diphtheria toxin-specific neutralizing activity. These results are the first demonstration that nasally co-administered mCT E112K can induce DT-specific protective Ab responses in mucosal compartments (e.g. lung lavages and the lungs).
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影响因子：
4.4
作者：
M. Marinaro;H. Staats;T. Hiroi;R. Jackson;M. Coste;P. Boyaka;N. Okahashi;M. Yamamoto;Hiroshi Kiyono;Horst Bluethmann;K. Fujihashi;Jerry R. McGhee
通讯作者：
M. Marinaro;H. Staats;T. Hiroi;R. Jackson;M. Coste;P. Boyaka;N. Okahashi;M. Yamamoto;Hiroshi Kiyono;Horst Bluethmann;K. Fujihashi;Jerry R. McGhee
人类对细菌内毒素的反应。
DOI：
--
发表时间：
1994
期刊：
Circulatory shock
影响因子：
--
作者：
R. Burrell
通讯作者：
R. Burrell
影响因子：
4.4
作者：
F. van Ginkel;J. Mcghee;C. Liu;J. Simecka;M. Yamamoto;R. Frizzell;E. Sorscher;H. Kiyono;D. Pascual
通讯作者：
F. van Ginkel;J. Mcghee;C. Liu;J. Simecka;M. Yamamoto;R. Frizzell;E. Sorscher;H. Kiyono;D. Pascual
内毒素诱导的气道炎症中淋巴细胞的剂量依赖性激活
影响因子：
3.1
作者：
R. Larsson;David Rocksén;B. Lilliehöök;Åsa Jonsson;A. Bucht
通讯作者：
A. Bucht
影响因子：
6.4
作者：
M. Vajdy;N. Lycke
通讯作者：
M. Vajdy;N. Lycke