Candida albicans strains heterozygous and homozygous for mutations in mitogen-activated protein kinase signaling components have defects in hyphal development
Candida albicans strains heterozygous and homozygous for mutations in mitogen-activated protein kinase signaling components have defects in hyphal development
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DOI：
10.1073/pnas.93.23.13223
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发表时间：
1996-11-12
影响因子：
11.1
通讯作者：
Fink, GR
中科院分区：
文献类型：
--
作者：
Kohler, JR;Fink, GR
白色念珠菌的基因，CST 20和HST 7，克隆他们的能力，以抑制交配缺陷的酿酒酵母突变体的ste 20和ste 7基因，其中代码的元素交配丝裂原活化蛋白（MAP）激酶途径。这些假丝酵母属基因是同源酵母属基因的结构和功能同源物。在酵母中的抑制模式与它们在MAP激酶级联中的假定位置有关。在念珠菌中构建了这些基因的等位基因。念珠菌纯合无效突变体在某些培养基上的菌丝形成有缺陷，但仍被血清诱导形成菌丝，表明血清诱导菌丝不依赖于MAP激酶级联。假丝酵母杂合子CST 20/cst 20和HST 7/hst 7在菌丝形成方面也有缺陷。这种野生型等位基因的优势的缺乏表明，基因剂量是重要的念珠菌。
The Candida albicans genes, CST20 and HST7, were cloned by their ability to suppress the mating defects of Saccharomyces cerevisiae mutants in the ste20 and ste7 genes, which code for elements of the mating mitogen-activated protein (MAP) kinase pathway. These Candida genes are both structural and functional homologs of the cognate Saccharomyces genes. The pattern of suppression in Saccharomyces is related to their presumptive position in the MAP kinase cascade. Null alleles of these genes were constructed in Candida. The Candida homozygous null mutants are defective in hyphal formation on some media, but are still induced to form hyphae by serum, showing that serum induction of hyphae is independent of the MAP kinase cascade. The Candida heterozygotes CST20/cst20 and HST7/hst7 are also defective in hyphal formation. This lack of dominance of the wild-type allele suggests that gene dosage is important in Candida.