Inhibition of epithelial cell apoptosis by Porphyromonas gingivalis
Inhibition of epithelial cell apoptosis by Porphyromonas gingivalis
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DOI：
10.1111/j.1574-6968.2001.tb10706.x
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发表时间：
2001-06-25
影响因子：
2.1
通讯作者：
Lamont, RJ
中科院分区：
文献类型：
--
作者：
Nakhjiri, SF;Park, Y;Lamont, RJ
Porphyromonas gingivalis is periodontal pathogen that is capable of invading gingival epithelial cells (GECs). Apoptotic responses of primary cultures of GECs to P. gingivalis were investigated with a DNA fragmentation ELISA assay. P gingivalis induced a transient increase in GEC DNA fragmentation: however. after prolonged incubation GECs did not undergo apoptosis. Furthermore, P. gingivalis blocked apoptosis in GECs following stimulation with camptothecin. Immunoblotting of GECs with Bcl-2 or Bax antibodies showed that P. gingivalis up-regulated Bcl-2 levels in GECs, whereas Bax levels were transiently elevated and declined after 24 h stimulation. Streptococcus gordonii did nor affect levels of either molecule. RT-PCR demonstrated that induction of Bcl-2 occurs at the transcriptional level. The results suggest that P. gingivalis can inhibit apoptosis. in GECs by up-regulation of the anti-apoptotic molecule Bcl-2. The prevention of host cell apoptosis may represent a strategy for P. gingivalis survival within invaded GECs. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
