Source of bacterial shedding in laminar flow theatres
Source of bacterial shedding in laminar flow theatres
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DOI：
10.1016/j.jhin.2004.06.028
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发表时间：
2004-11-01
影响因子：
6.9
通讯作者：
Bannister, GC
中科院分区：
文献类型：
--
作者：
Owers, KL;James, E;Bannister, GC
在之前未发表的观察结果中，在层流手术室进行关节成形术期间，56个裂隙空气样本中有2个（3.57%）发生了不可接受的高细菌计数，可能是由于脱落事件。分离株与两次手术中从其中一名外科医生前额皮肤上采集的拭子样本中培养的分离株相匹配。尽管洗手间工作人员穿着标准的封闭式长袍、戴着帽子和面罩，但仍发生了这些发现。为了更准确地定位这种脱落的潜在来源，20名剧院工作人员从剧院服装未覆盖的面部区域（即额头、眉毛和耳朵）进行了匿名微生物擦拭。对这些拭子进行培养，并对生长情况进行统计学比较。与研究的其他两个面部区域相比，从剧院工作人员的耳朵采集的拭子中培养出的菌落显著更多（P = 0.047，Freidman检验）。这些数据支持在关节成形术中使用排气头盔，或者至少强制手术室工作人员用手术帽覆盖耳朵。(C)2004年医院感染学会。由爱思唯尔有限公司出版。保留所有权利。
In a previous unpublished observation, unacceptably high bacterial, counts, presumably due to shedding episodes, occurred in two of 56 (3.57%) slit-air samples during arthroplasty surgery in a laminar flow operating theatre. The isolates matched those cultured from swab samples taken from the skin of one of the surgeons's foreheads on each of the two occasions. These findings occurred despite scrub staff wearing standard occlusive gowns, hats and masks with visors. In order to localize the potential source of such shedding more accurately, 20 members of theatre staff underwent anonymous microbiological swabbing from the facial areas not covered by theatre clothing, namely their foreheads, eyebrows and ears. These swabs were cultured and the growths were compared statistically. Significantly more colonies were cultured from swabs taken from the theatre staff's ears (P = 0.047, Freidman's test) compared with the other two facial areas studied. These data support the use of exhaust helmets in arthroplasty surgery, or at least mandatory coverage of the ears with theatre hats for scrub staff. (C) 2004 The Hospital Infection Society. Published by Elsevier Ltd. All rights reserved.