Overexpression of TaSIM provides increased drought stress tolerance in transgenic Arabidopsis
Overexpression of TaSIM provides increased drought stress tolerance in transgenic Arabidopsis
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TaSIM 的过度表达提高了转基因拟南芥的干旱胁迫耐受性
DOI：
10.1016/j.bbrc.2019.03.007
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发表时间：
2019
影响因子：
3.1
通讯作者：
Ni Zhiyong
中科院分区：
文献类型：
--
作者：
Yu Yuehua;Bi Chenxi;Wang Qing;Ni Zhiyong
干旱是影响小麦生产的最严重的气象灾害。R2R3-.MYB基因亚家族成员在小麦干旱胁迫反应的调控中起着至关重要的作用。本研究探讨了聚乙二醇诱导小麦R2R3-MYB基因TaSIM.在干旱胁迫下表达的功能。β-葡萄糖醛酸苷酶(GUS)组织化学染色表明，Tasim启动子可调控GUS基因在花、根、茎和莲座叶中的表达。此外，Tasim在小麦的雄蕊、雌蕊、根、茎和叶中都有表达。TaSIM启动子含有一个已知的胁迫反应顺式作用元件，可被胁迫、PEG和脱落酸(ABA)诱导。干旱胁迫下，与野生型(WT)拟南芥相比，转Tasim基因拟南芥的叶片失水率显著降低，成活率显著提高，可溶性糖和脯氨酸含量以及胁迫相关基因(RD29A和RD22)的表达水平均高于野生型拟南芥。结果表明，Tasim基因在干旱胁迫响应中起积极作用，可作为小麦抗旱基因工程的候选基因。
Drought is the most serious meteorological disaster affecting wheat production. Members of the R2R3-.MYB gene subfamily play a crucial role in the regulation of the wheat drought stress response. In this.study, the function of polyethylene glycol (PEG)-induced expression of the wheat R2R3-MYB gene TaSIM.in response to drought stress was characterized. b-Glucuronidase (GUS) histochemical staining revealed.that the TaSIM promoter can drive the expression of the GUS gene in the flowers, roots, stems and rosette.leaves. Moreover, TaSIM was expressed in the stamens, pistils, roots, stems and leaves of wheat. The.TaSIM promoter contains a known stress-responsive cis-acting element and is inducible by stress, PEG.and abscisic acid (ABA). Under drought stress, compared with wild-type (WT) Arabidopsis, transgenic.Arabidopsis overexpressing TaSIM presented significantly lower leaf water loss rates and increased survival..Moreover, the content of soluble sugars and proline and the expression of stress-related genes.(RD29A and RD22) in transgenic Arabidopsis overexpressing TaSIM were higher than those in WT Arabidopsis.under drought stress. Our results indicate that TaSIM plays a positive role in the drought stress.response and can be used as a candidate gene for the genetic engineering of wheat drought resistance.