Alteration in gut microbiota associated with hepatitis B and non-hepatitis virus related hepatocellular carcinoma
Alteration in gut microbiota associated with hepatitis B and non-hepatitis virus related hepatocellular carcinoma
复制标题
与乙型肝炎和非肝炎病毒相关的肝细胞癌相关的肠道微生物群的改变
DOI：
10.1186/s13099-018-0281-6
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发表时间：
2019-01-18
期刊：
影响因子：
4.2
通讯作者：
Liu, Xingyin
中科院分区：
文献类型：
--
作者：
Liu, Qisha;Li, Fan;Liu, Xingyin
背景肝细胞癌（HCC）的发病在世界恶性肿瘤中排名第五。越来越多的证据表明，HCC的分布与慢性乙型肝炎病毒（HBV）感染的发生率以及酒精中毒、黄曲霉毒素B1摄入和肥胖等因素有关。最近的研究表明，肠道生态失调在肝脏疾病中起着重要作用。然而，HBV和非HBV非hcv相关HCC的肠道菌群研究尚未见报道。在本研究中，我们研究了HBV相关HCC （B-HCC）和非HBV非hcv相关HCC （NBNC-HCC）肠道微生物群的差异，最终发现了一些潜在的细菌，将两种类型HCC的不同病理机制联系起来。结果我们对33名健康对照、35名HBV相关HCC （B-HCC）患者和22名非HBV非hcv （NBNC）相关HCC （NBNC-HCC）患者进行了16S rRNA分析。我们发现B-HCC患者粪便微生物群的物种丰富度远高于其他两组。有趣的是，NBNC-HCC患者的粪便中含有更多潜在的促炎细菌（埃希氏志贺氏菌、肠球菌），粪杆菌、瘤胃球菌、瘤胃杆菌水平降低，导致抗炎短链脂肪酸的潜力降低。NBNC-HCC患者粪便中与氨基酸和葡萄糖代谢相关的多种生物学通路丰度相对较少，但部分类型的转运和分泌水平较高。然而，B-HCC患者的细菌组成和相关的多种生物学途径的结果与NBNC-HCC患者相反。同时，我们发现B-HCC和NBNC-HCC患者肠道微生物群异常网络的发生方式不同。结论我们的研究表明，B-HCC和NBNC-HCC患者中参与不同功能或生物学途径的细菌丰度存在差异。我们认为特异性肠道菌群的改变可能为B-HCC和NBNC-HCC提供治疗益处。
BackgroundThe onset of hepatocellular carcinoma (HCC) ranked fifth malignancies all over the world. Increasing evidences showed that the distribution of HCC was related to the incidence of chronic hepatitis B virus (HBV) infection and other factors, such as alcoholism, aflatoxin B1 ingestion and obesity. Recent studies demonstrated that gut dysbiosis plays an important role in liver diseases. However, the researches on gut microbiota of HBV and non-HBV non-HCV related HCC have not been reported. In this study, we investigated the differences between the gut microbiota of HBV related HCC (B-HCC) and non-HBV non-HCV related HCC (NBNC-HCC), finally found some potential bacteria, linking different pathological mechanism of both types of HCCs.ResultsWe carried out 16S rRNA analyses in a cohort of 33 healthy controls, 35 individuals with HBV related HCC (B-HCC) and 22 individuals with non-HBV non-HCV (NBNC) related HCC (NBNC-HCC). We found that the species richness of fecal microbiota of B-HCC patients was much higher than other two groups. Interestingly, the feces of NBNC-HCC patients harbored more potential pro-inflammatory bacteria (Escherichia-Shigella, Enterococcus) and reduced levels of Faecalibacterium, Ruminococcus, Ruminoclostridium which results in decrease potential of anti-inflammatory short-chain fatty acids. The feces of NBNC-HCC patients had relatively fewer abundance of multiple biological pathways related to amino acid and glucose metabolism, but high level of transport and secretion in some types. However, the B-HCC patients had opposite results of bacterial composition and associated multiple biological pathways versus NBNC-HCC patients. Meanwhile, we found that aberrant network of gut microbiota occurred differently in B-HCC and NBNC-HCC patients.ConclusionsOur study indicated that B-HCC and NBNC-HCC patients showed differential abundance of bacteria involved in different functions or biological pathways. We suggested the modification of specific gut microbiota may provide the therapeutic benefit for B-HCC and NBNC-HCC.