On/off control of biomolecular motors in a microfluidic device
On/off control of biomolecular motors in a microfluidic device
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微流体装置中生物分子马达的开/关控制
DOI：
10.1109/sensor.2003.1216968
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
H. Fujita
中科院分区：
文献类型：
--
作者：
R. Yokokawa;S. Takeuchi;T. Kon;M. Nishiura;M. Edamatsu;K. Sutoh;H. Fujita
我们已经成功地实现了生物分子直线电机的开/关控制在聚二甲基硅氧烷（PDMS）密封的玻璃罩室。一个线性的生物分子运动系统，微管运动系统，通过注射优化浓度的三磷酸腺苷（ATP）来激活和己糖激酶来停止。不仅仅是控制从开到关或从开到开，ATP和己糖激酶的重复注射证明，一旦激酶分子与微管结合，它们可以交替地移动和停止。
We have successfully realized an on/off control of biomolecular linear motors in a polydimethyl siloxane (PDMS) chamber sealed with a cover glass. A linear biomolecular motor system, microtubule-kinesin system, is controlled by injecting the optimized concentrations of adenosine triphosphate (ATP) for the activation and hexokinase for the stoppage. Not only control from on to off or off to on, the repetitive injection of ATP and hexokinase proves that kinesin molecules can move and stop alternatively once they bind to microtubules.