Tumours acquire their vasculature by vessel incorporation, not vessel ingrowth
Tumours acquire their vasculature by vessel incorporation, not vessel ingrowth
复制标题
肿瘤通过血管合并而不是血管向内生长获得脉管系统
作者：
W. Thompson;K. J. Shiach;R. Fraser;L. McIntosh;J. G. Simpson
本研究旨在利用可移植的小鼠乳腺癌来研究肿瘤循环的早期发展。将肿瘤细胞 (106) 皮下注射到胁腹，并每隔 24 小时杀死一组接受治疗的小鼠，最多持续 12 天；通过标准组织学方法处理肿瘤和周围组织。使用计算机辅助形态测量技术将肿瘤切片细分为肿瘤组织、血管和结缔组织：肿瘤周围的宿主组织对血管和结缔组织进行了类似的量化。随着肿瘤大小的增加，肿瘤内血管的比例迅速增加，达到肿瘤体积约 1·5% 的稳定状态，比正常皮下组织的血管密度增加了 400%。在肿瘤内，外围的血管密度总是高于中心。血管密度最明显的增加影响了肿瘤周围的宿主组织。目前还不清楚为什么当假设的血管生成因子（例如肿瘤血管生成因子）可能在肿瘤内部释放时，血管发育在肿瘤外最为突出。然而，我们的结果表明，肿瘤通过浸润到周围结缔组织中新形成的血管网络并在其之间扩张来获得其脉管系统。
This study was designed to investigate the early development of tumour circulation using a transplantable mouse mammary adenocarcinoma. Tumour cells (106) were injected subcutaneously into the flank and groups of treated mice were killed at 24 h intervals up to 12 days; the tumours and surrounding tissues being processed by standard histological methods. Sections of tumour were sub‐divided into neoplastic tissue, vessels and connective tissue using computer‐assisted morphometric techniques: the host tissue around the tumour was similarly quantified for vessels and connective tissue. With increasing tumour size, the proportion of vessels within tumours rapidly increased to reach a plateau of approximately 1·5 per cent of tumour volume, a 400 per cent increase on the vascular density of normal subcutaneous tissue. Within tumours, vascular density was always higher at the periphery than the centre. The most pronounced increase in vascular density affected the host tissue around the tumour. It is not clear why vascular development is most prominent outwith the tumour when postulated angiogenic factors, such as tumour angiogenesis factor, are presumably released within. Our results imply, however, that tumours acquire their vasculature by infiltration into, and expansion between, a network of newly formed vessels in the surrounding connective tissue.
DOI：
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发表时间：
1984-12
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
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作者：
P. Polverini;S. Leibovich
通讯作者：
P. Polverini;S. Leibovich