ELECTROSPRAY IONIZATION MASS-SPECTROMETRIC PEPTIDE-MAPPING - A RAPID, SENSITIVE TECHNIQUE FOR PROTEIN-STRUCTURE ANALYSIS
ELECTROSPRAY IONIZATION MASS-SPECTROMETRIC PEPTIDE-MAPPING - A RAPID, SENSITIVE TECHNIQUE FOR PROTEIN-STRUCTURE ANALYSIS
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DOI：
10.1016/0006-291x(90)92080-j
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发表时间：
1990-03-16
影响因子：
3.1
通讯作者：
CHAIT, BT
中科院分区：
文献类型：
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作者：
CHOWDHURY, SK;KATTA, V;CHAIT, BT
电喷雾电离质谱肽图谱是一种有用的蛋白质结构分析新技术。这个过程包括用胰酶消化蛋白质，然后用电喷雾电离质谱仪分析总的未分离的消化液。以人载脂蛋白AI(MR=28 kDa)的肽图分析为例，说明了该技术在蛋白质结构研究中的应用。该技术快速、灵敏，不需要事先分离多肽。在本程序中，在其他质谱学方法中观察到的辨别效应不那么重要。
Electrospray ionization mass spectrometric peptide mapping is demonstrated to be a useful new technique for protein structure analysis. The procedure involves the digestion of the protein with trypsin and subsequent analysis of the total unfractionated digest by electrospray ionization mass spectrometry. The utility of the technique for investigating protein structure is illustrated by a peptide mapping analysis of human apolipoprotein AI (Mr = 28 kDa). The technique is rapid, sensitive, and requires no prior separation of the peptides. The discrimination effects observed in other mass spectrometric methods are less important in the present procedure.