Genetic Diversity of Daphnia pulex in the Middle and Lower Reaches of the Yangtze River.
Genetic Diversity of Daphnia pulex in the Middle and Lower Reaches of the Yangtze River.
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长江中下游水蚤遗传多样性
DOI：
10.1371/journal.pone.0152436
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Xu X
中科院分区：
文献类型：
--
作者：
Wang W;Zhang K;Deng D;Zhang YN;Peng S;Xu X
人类活动的增加和环境的变化可能会导致水中枝角类的遗传多样性变化。普氏水蚤分布于世界各地，常被视为模式生物。以16S rDNA、细胞色素C氧化酶亚基I(COI)和18S基因为分子标记。对采自长江中下游10个水体的普氏杜鹃的遗传多样性和系统发育进行了研究。16S rDNA、COI基因和18S基因的A+T含量(65.4%、58.4%和54.6%)显著高于G+C含量(34.6%、41.6%和45.4%)。这一结果与无脊椎动物中较高的A和T含量一致。根据16S rDNA和coi基因的遗传距离，来自长江中下游中国段的10个水体中的白纹伊蚊的遗传距离最小(分别为0%~0.8%和0%~1.5%)。而在系统发育树上，普氏杜鹃进化成两个分支，这与其地理分布相吻合。与国外比较，长江中下游10个水体的平均遗传距离达9.1%~10.5%，提示可能进化为不同的亚种。
Increased human activities and environmental changes may lead to genetic diversity variations of Cladocerans in water. Daphnia pulex are distributed throughout the world and often regarded as a model organism. The 16S rDNA, cytochrome c oxidase subunit I (COI), and 18S genes were used as molecular marks. The genetic diversity and phylogeny of D. pulex obtained from 10 water bodies in the middle and lower reaches of the Yangtze River were studied. For 16S rDNA, COI gene, and 18S gene, the A+T content (65.4%, 58.4%, and 54.6%) was significantly higher than the G+C content (34.6%, 41.6% and 45.4%). This result was consistent with higher A and T contents among invertebrates. Based on the genetic distances of 16S rDNA and COI genes, the genetic differences of D. pulex from 10 water bodies located in the middle and lower reaches of the Yangtze River in China was minimal (0%–0.8% for 16S rDNA and 0%–1.5% for COI gene). However, D. pulex evolved into two branches in the phylogenetic trees, which coincided with its geographical distribution. Compared with D. pulex from other countries, the average genetic distance of D. pulex obtained from 10 water bodies in the middle and lower reaches of the Yangtze River reached 9.1%–10.5%, thereby indicating that D. pulex may have evolved into different subspecies.