A Versatile Synthetic Extracellular Matrix Mimic via Thiol-Norbornene Photopolymerization.
A Versatile Synthetic Extracellular Matrix Mimic via Thiol-Norbornene Photopolymerization.
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DOI：
10.1002/adma.200901808
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发表时间：
2009-12-28
影响因子：
29.4
通讯作者：
Anseth, Kristi S
中科院分区：
文献类型：
--
作者：
Fairbanks, Benjamin D;Schwartz, Michael P;Halevi, Alexandra E;Nuttelman, Charles R;Bowman, Christopher N;Anseth, Kristi S
具有工程化的、细胞介导的降解位点的合成水凝胶是一类重要的仿生材料。在此，水凝胶是通过自由基介导的硫醇 - 降冰片烯（硫醇 - 烯）光聚合的逐步增长反应机制合成的。该反应将理想的、均匀的聚合物网络形成、无需合成后修饰即可轻松引入肽以及对网络演变的时空控制等优点结合到一个单一系统中，以生产可被蛋白水解降解的聚乙二醇（PEG）肽水凝胶。利用硫醇 - 烯光聚合，实现了快速凝胶化时间，同时在细胞包封时保持了高细胞活力。通过选择可被蛋白水解降解的交联剂以及黏附肽RGDS的密度来调整人间充质干细胞（hMSCs）的扩散速率，证明了酶和细胞响应特性。此外，通过生化光图案化在硫醇 - 烯水凝胶内对细胞功能进行空间操控。对凝胶化的高度时空控制，结合稳健的材料性能，使得硫醇 - 烯水凝胶成为多种医学和生物学应用的极佳工具。
Synthetic hydrogels with engineered, cell-mediated degradation sites are an important category of biomimetic materials. Here, hydrogels are synthesized by a step-growth reaction mechanism via a radically mediated thiol-norbornene (thiol-ene) photopolymerization. This reaction combines the advantages of ideal, homogeneous polymer network formation, facile incorporation of peptides without post-synthetic modification, and spatial and temporal control over the network evolution into a single system to produce proteolytically degradable poly(ethylene glycol) (PEG) peptide hydrogels. Using a thiol-ene photopolymerization, rapid gelation times are achieved, while maintaining high cell viability for cell encapsulation. The enzyme- and cellresponsive characteristics are demonstrated by tailoring the rate of spreading of human mesenchymal stem cells (hMSCs) through both the selection of proteolytically degradable crosslinkers and the density of the adhesion peptide RGDS. Furthermore, cellular function is manipulated spatially within the thiol-ene hydrogels through biochemical photopatterning. The high degree of spatial and temporal control over gelation, combined with robust material properties, makes thiol-ene hydrogels an excellent tool for a variety of medical and biological applications.