Effect of vasopressin as a local anesthetic in mice
Effect of vasopressin as a local anesthetic in mice
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DOI：
10.1007/s10266-020-00491-2
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发表时间：
2020-02-10
期刊：
影响因子：
2.5
通讯作者：
Sunda, Katsuhisa
中科院分区：
文献类型：
--
作者：
Katagiri, Keiko;Hashimoto, Shuichi;Sunda, Katsuhisa
我们试图阐明如何通过尾部局部给药盐酸甲哌卡因和加压素影响外周血流量，组织动力学，和甲哌卡因的麻醉效果在小鼠。本研究使用了226只雄性ICR小鼠。通过尾部组织单独给予甲哌卡因或甲哌卡因与0.03、0.3或3.0 U/mL加压素或10 μ g/mL肾上腺素一起给药后，测量血流量。用H-3标记的盐酸甲哌卡因与加压素或肾上腺素一起测量尾部组织和血液动力学。甲哌卡因与0.3U/mL加压素联合应用后，测定复合神经动作电位（CNAP）以阐明麻醉效果。采用的统计方法为Steel-Dwass检验、Mann-Whitney U检验、Dunnett检验和Tukey检验。P < 0.05 indicated statistical significance. The results revealed that the local administration of >= 0.03 U/mL加压素以浓度依赖性方式减少局部血流量并延长尾部组织中的H-3-M定位。0.3U/mL加压素可增强和延长甲哌卡因的麻醉作用。研究结果表明，在局部麻醉方案中加入加压素可能是有效的，因此它可以作为血管收缩剂应用。
We sought to elucidate how the local administration of mepivacaine hydrochloride and vasopressin via the tail affects the peripheral blood flow volume, tissue dynamics, and mepivacaine's anesthetic effect in mice. Two-hundred and twenty-six male ICR mice were used in this study. Blood flow was measured after administering mepivacaine alone or mepivacaine with either 0.03, 0.3, or 3.0 U/mL vasopressin or 10 mu g/mL epinephrine via the tail tissue. The tail tissue and blood dynamics were measured using H-3-labeled mepivacaine hydrochloride with vasopressin or epinephrine. The compound nerve action potential (CNAP) was measured to clarify the anesthetic effect after administering mepivacaine with 0.3 U/mL vasopressin. The statistical methods employed were Steel-Dwass test, Mann-Whitney U test, Dunnett's test, and Tukey test. P < 0.05 indicated statistical significance. The results revealed that the local administration of >= 0.03 U/mL vasopressin reduced local blood flow and prolonged H-3-M localization in the tail tissue in a concentration-dependent manner. Addition of 0.3 U/mL vasopressin enhanced and prolonged the anesthetic effect of mepivacaine. The findings suggest that adding vasopressin to a local anesthetic regimen may be effective, and thus it could be applied as a vasoconstrictor.