Matrix metalloproteinases in carcinoma of unknown primary
Matrix metalloproteinases in carcinoma of unknown primary
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DOI：
10.1002/cncr.21454
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发表时间：
2005-11-15
期刊：
影响因子：
6.2
通讯作者：
Pavlidis, N
中科院分区：
文献类型：
--
作者：
Karavasilis, V;Malamou-Mitsi, V;Pavlidis, N
背景目的：研究原发性不明癌（CUP）中蛋白水解相关分子基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶-9（MMP-9）及金属蛋白酶组织抑制剂-1（TIMP-1）的表达。使用了来自75名诊断为CUP的患者的石蜡包埋的肿瘤材料。肿瘤组织学类型为腺癌（77%）、未分化癌（19%）和鳞状细胞癌（4%），患者分为有利（62%）和不利（38%）亚组。MMP-2、MMP-9和TIMP-1的组织表达通过使用特异性单克隆抗体进行评估，并通过视觉染色评分进行评价。研究分子的表达进行了分析，对临床病理数据。MMP-2在69%（强表达49%）、MMP-9在49%（强表达36%）、TIMP-1在79%（强表达44%）的研究病例中被发现表达。MMP-2和MMP-9的表达呈正相关。与良性肿瘤相比，不良肿瘤中TIMP-1显著更高，并且与患者的生存期更短相关（7.5 vs. 12个月）。未发现其他相关性。MMP-2、MMP-9和TIMP-1在CUP中广泛表达，提示蛋白水解在这些肿瘤中起重要作用。TIMP-1可能是CUP患者预后不良的一个标志物。
BACKGROUND. The purpose was to study proteolysis-related molecules, matrix metalloproteinase-2 (MMP-2) and MMP-9 and tissue inhibitor of metalloproteinases-1 (TIMP-1), in carcinoma of unknown primary (CUP).METHODS. Paraffin-embedded tumor material from 75 patients diagnosed with CUP was used. Tumor histologies were adenocarcinoma (77%), undifferentiated carcinoma (19%), and squamous cell carcinoma (4%) and patients were categorized into favorable (62%) and unfavorable (38%) subsets. The tissue expression of MMP-2, MMP-9, and TIMP-1 was assessed by use of specific monoclonal antibodies and evaluated by means of a visual staining score. The expression of molecules studied was analyzed against clinicopathological data.RESULTS. MMP-2 was found expressed in 69% (strong expression in 49%), MMP-9 in 49% (strong in 36%), and TIMP-1 in 79% (strong in 44%) of studied cases. The expression of MMP-2 correlated positively with MMP-9. TIMP-1 was significantly higher in unfavorable compared with favorable tumors and was associated with a shorter survival of patients (7.5 vs. 12 mos). No other associations were detected.CONCLUSIONS. MMP-2, MMP-9, and TIMP-1 are widely expressed in CUP, suggesting an essential role of proteolysis in these tumors. TIMP-1 may be considered a possible marker of poor prognosis in CUP patients.