Modulation of Anopheles gambiae Epsilon glutathione transferase activity by plant natural products in vitro
Modulation of Anopheles gambiae Epsilon glutathione transferase activity by plant natural products in vitro
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DOI：
10.1080/14756360701546595
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发表时间：
2008-01-01
影响因子：
5.6
通讯作者：
Mukanganyama, Stanley
中科院分区：
文献类型：
--
作者：
Muleya, Victor;Hayeshi, Rose;Mukanganyama, Stanley
Elevated glutathione transferase (GST) E2 activity is associated with DDT resistance in the mosquito Anopheles gambiae. The search for chemomodulators that inhibit the function of AgGSTE2 would enhance the insecticidal activity of DDT. Therefore, we examined the interaction of novel natural plant products with heterologously expressed An. gambiae GSTE2 in vitro. Five of the ten compounds, epiphyllocoumarin(Tral-1), knipholone anthrone, isofuranonaphthoquinones (Mr 13/2, Mr 13/4) and the polyprenylated benzophenone (GG1) were shown to be potent inhibitors of AgGSTE2 with IC50 values of 1.5 mu M, 3.5 mu M, 4 mu M, 4.3 mu M and 4.8 mu M respectively. Non-competitive inhibition was obtained for Tral 1 and GG1 with regards to GSH (K-i of 0.24 mu M and 0.14 mu M respectively). Competitive inhibition for Tral1 was obtained with CDNB (K-i = 0.4 mu M) whilst GG1 produced mixed type of inhibition. The K-i and K-i' for GSH for Tral-1 and GG1 were 0.2 mu M and 0.1 mu M respectively. These results suggest that the novel natural plant products, particularly Tral-1, represent potent AgGSTE2 in vitro inhibitors.
