MIN6 Is Not a Pure Beta Cell Line but a Mixed Cell Line with Other Pancreatic Endocrine Hormones
MIN6 Is Not a Pure Beta Cell Line but a Mixed Cell Line with Other Pancreatic Endocrine Hormones
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DOI：
10.1507/endocrj.k08e-172
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发表时间：
2009-02-01
影响因子：
2
通讯作者：
Kaku, Kohei
中科院分区：
文献类型：
--
作者：
Nakashima, Koji;Kanda, Yukiko;Kaku, Kohei
作为分离的胰岛，MIN6细胞保留葡萄糖刺激胰岛素分泌（GSIS）。我们综合评估了MIN6细胞中其他胰岛激素的基因表达和产生。胰岛激素采用免疫组化染色，ELISA检测。将MIN6细胞的基因表达谱与激光捕获显微解剖（LCM）获得的小鼠胰岛细胞进行比较。MIN6细胞分泌胰岛素、胰高血糖素、生长抑素和胃饥饿素。它们表达了胰岛素I和II、胰高血糖素原、生长抑素、胰多肽（PP）和胃饥饿素的mrna，这些mrna在小鼠胰岛核心和周围显示。多种与胰岛激素产生细胞密切相关的基因在MIN6中表达。共聚焦激光扫描显微镜观察发现，MIN6细胞不仅包括胰岛素阳性细胞，还包括胰岛素和胰高血糖素或生长激素双阳性细胞。培养基中检测到胰高血糖素、生长抑素和胃饥饿素。本研究清楚地证明了MIN6产生胰腺内分泌细胞。该细胞系有可能作为研究胰岛发育、细胞分化和功能的模型。
MIN6 cells retains glucose-stimulated insulin secretion (GSIS) as isolated islets. We comprehensively evaluated the gene expression and production of other islet hormones in MIN6 cells. Islet hormones were demonstrated by immunohistochemical staining and measured by ELISA. The gene expression profiles of MIN6 cells were compared with those in the Mouse islets obtained by the laser capture micro-dissection (LCM). MIN6 cells excreted insulin, glucagon, somatostatin and ghrelin. They expressed mRNAs of insulin I and II, proglucagon, somatostatin, pancreatic polypeptide (PP) and ghrelin which were shown in the mouse pancreatic islet core and periphery obtained by LCM. A variety of genes closely related to the islet hormone producing cells were expressed in MIN6. Confocal laser scanning microscopy revealed that MIN6 cells included not only insulin positive cells but also insulin and glucagon or somatostin double positive cells. Glucagon, somatostatin and ghrelin were detectable in the culture medium. The present study clearly demonstrated that MIN6 produce pancreatic endocrine cells. It would be possible to use this cell line as a model to research the development, cell differentiation and function of pancreatic islets.