High-frequency cotransfer of the transformed phenotype and a tumor-specific transplantation antigen by DNA from the 3-methylcholanthrene-induced Meth A sarcoma of BALB/c mice.
High-frequency cotransfer of the transformed phenotype and a tumor-specific transplantation antigen by DNA from the 3-methylcholanthrene-induced Meth A sarcoma of BALB/c mice.
复制标题
来自 3-甲基胆蒽诱导的 BALB/c 小鼠 Meth A 肉瘤的 DNA 高频共转移转化表型和肿瘤特异性移植抗原。
DOI：
10.1073/pnas.78.12.7555
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发表时间：
1981
影响因子：
11.1
通讯作者：
Law,LW
中科院分区：
文献类型：
--
作者：
Hopkins,N;Besmer,P;DeLeo,AB;Law,LW
We transformed BALB/3T3 mouse cells with cellular DNA extracted from the Meth A sarcoma, a 3-methylcholanthrene-induced tumor of BALB/c mice, and asked whether foci arising in the transfection possess the previously defined Meth A tumor-specific transplantation antigen (TSTA). Five of eight foci selected from one experiment possessed Meth A TSTA. DNA extracted from one of the five TSTA-positive clones was used in secondary rounds of transfection transformation. Four out of five foci tested from the secondary transfections possessed Meth A TSTA. These results suggest that in the Meth A sarcoma a transforming gene and a genetic determinant of TSTA are intimately related: they may be identical or very closely linked; alternatively, a particular transforming gene might induce the expression of a particular TSTA. Another possible explanation for these results is that the cotransfer of certain cellular genes by DNA transfection is considerably higher than predicted from the limited studies presently available.
