High-risk human papillomavirus E7 oncoprotein detection in cervical squamous cell carcinoma
High-risk human papillomavirus E7 oncoprotein detection in cervical squamous cell carcinoma
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DOI：
10.1158/1078-0432.ccr-07-1222
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发表时间：
2007-12-01
影响因子：
11.5
通讯作者：
Zwerschke, Werner
中科院分区：
文献类型：
--
作者：
Ressler, Sigrun;Scheiden, Rene;Zwerschke, Werner
目的：高危型人乳头瘤病毒（HPV）持续感染是宫颈癌的主要病因。本研究的目的是评估高危E7癌蛋白是否足以作为宫颈癌检测的标志物。实验设计：对58个宫颈癌和22个正常宫颈鳞状上皮的活检样本进行HPV分型。通过免疫组织化学监测 HPV-16 E7、HPV-18 E7 和 HPV-45 E7 癌蛋白水平，并与 p16(INK4a) 和 Ki67 进行比较。结果：55 个 (94.8%) 肿瘤为高危 HPV-DNA 阳性（46 个 HPV-16, 2 个 HPV-16 和 HPV-18, 4 个） HPV-18、1 HPV-33 和 2 HPV-45）。在三个肿瘤（5.2%）中未检测到 HPV-DNA。 57 种宫颈癌 (98.3%) 中显示出高 HPV E7 癌蛋白水平，但表达水平与肿瘤分期之间没有相关性。结论：这是第一项系统分析宫颈癌中主要 HPV 癌蛋白水平的研究，表明高危 HPV E7 蛋白在这些癌症中规律表达。这表明高风险 E7 癌蛋白是宫颈癌所必需的，并且作为肿瘤标志物显然是必不可少的。
Purpose: Persistent infections by high-risk human papillomavirus (HPV) types are the main etiologic factor for cervical cancer. The objective of this study was to evaluate whether high-risk E7 oncoprotein is adequate as a marker for the detection of cervical cancer.Experimental Design: HPV typing was done in biopsies from 58 cervical carcinoma and 22 normal cervical squamous epithelia. The HPV-16 E7, HPV-18 E7, and HPV-45 E7 oncoprotein levels were monitored by immunohistochemistry and compared with those of p16(INK4a) and Ki67.Results: Fifty-five (94.8%) tumors were high-risk HPV-DNA-positive (46 HPV-16, 2 HPV-16 and HPV-18, 4 HPV-18,1 HPV-33, and 2 HPV-45). HPV-DNA could not be detected in three tumors (5.2%). High HPV E7 oncoprotein levels were shown in 57 cervical cancers (98.3%), without correlation between expression levels and tumor stages.Conclusion: This is the first study which systematically analyzes the levels of the major HPV oncoproteins in cervical carcinomas demonstrating that the high-risk HPV E7 proteins are regularly expressed in these cancers. This suggests that high-risk E7 oncoproteins are necessary for cervical cancers and apparently essential as tumor marker.