Chemiluminescence-based aptasensors for various target analytes
Chemiluminescence-based aptasensors for various target analytes
复制标题
用于各种目标分析物的基于化学发光的适体传感器
DOI：
10.1002/bio.3557
复制
发表时间：
2018
期刊：
影响因子：
2.9
通讯作者：
Lu Jianzhong
中科院分区：
文献类型：
--
作者：
Sun Yue;Lu Jianzhong
适体（DNA或RNA）具有复杂的三维形状，可以结合特定的靶标。相对于抗体，适体受益于它们的低生产成本、容易的化学修饰、高化学稳定性、再现性以及低水平的免疫原性和毒性。然而，适体的真正价值在于它们的简单性，这些分子可以被设计成传感器，作为诊断，药物发现和治疗，环境监测和食品质量检测等中的生物识别元件。许多不同类型的技术，如光学，电化学，放射化学和压电方法，已被应用于基于适体的方法的设计，其中化学发光（CL）检测技术近年来变得非常流行。本文综述了近年来基于适体的化学发光传感器在不同靶点检测方面的研究进展。我们强调了具体的例子，展示了在实际应用中使用的适体，并提供了在这个有前途的领域的挑战和机遇。
Aptamers (DNA or RNA) have complex three‐dimensional shapes that can bind to specific targets. Relative to antibodies, aptamers benefit from their low cost of production, easy chemical modification, high chemical stability, reproducibility, and low levels of immunogenicity and toxicity. However, the true value of aptamers lies in their simplicity by which these molecules can be engineered into sensors as bio‐recognition elements in diagnostics, drug discovery and therapy, environmental monitoring and food quality testing, etc. Many different types of techniques, such as optical, electrochemical, radiochemical and piezoelectronic methods, have been applied for the design of aptamer‐based methods, in which chemiluminescence (CL) detection techniques have become very popular in recent years. This review focuses on the recent advances in the development of aptamer‐based CL sensors for different target detection. We highlight specific examples that showcase the use of aptamers in practical applications, and provide the challenges and opportunities in this promising field.