Effects of fermentation products of pro- and prebiotics on trans-epithelial electrical resistance in an in vitro model of the colon
Effects of fermentation products of pro- and prebiotics on trans-epithelial electrical resistance in an in vitro model of the colon
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DOI：
10.1207/s15327914nc5101_14
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发表时间：
2005-01-01
影响因子：
2.9
通讯作者：
Rowland, IR
中科院分区：
文献类型：
--
作者：
Commane, DM;Shortt, CT;Rowland, IR
来自体内和体外研究的证据表明，益生元和益生元的消费可能会抑制结肠癌的发生；然而，到目前为止，相关的机制被证明是难以捉摸的。有一些动物研究的迹象表明，这种作用是在致癌的促进阶段发挥的。结直肠癌推进阶段的一个特征是紧绷的功能被破坏，导致跨越肠道屏障的完整性丧失。我们使用Caco-2人腺癌细胞系作为肠上皮细胞的模型。跨上皮电阻测量表明Caco-2单层完整性，我们记录了在接触选定的益生菌和益生菌的发酵产品后，这种完整性的变化，以不可消化的寡糖(NDO)的形式。我们的结果表明，NDO本身对紧密连接的强度有不同的影响，但通常影响很小，而益生菌和NDOS的发酵产物往往会提高紧密连接的完整性。这种作用是细菌种类和低聚糖专一性的。双歧杆菌BB12尤其有效，雷公藤红素和雷公藤红素的发酵产物也是如此。我们进一步研究了Raftiose发酵对脱氧胆酸(DCA)对紧密功能完整性的负面影响的保护能力。我们发现保护作用是物种依赖的，依赖于在接触DCA的同时或之后发酵产物在介质中的存在。结果表明，雷公藤红素发酵产物可以防止肿瘤促进剂损伤时肠上皮屏障功能的破坏。
Evidence from in vivo and in vitro studies suggests that the consumption of pro- and prebiotics may inhibit colon carcinogenesis; however, the mechanisms involved have, thus far proved elusive. There are some indications from animal studies that the effects are being exerted during the promotion stage of carcinogenesis. One feature of the promotion stage of colorectal cancer is the disruption of tight functions, leading to a loss of integrity across the intestinal barrier. We have used the Caco-2 human adenocarcinoma cell line as a model for the intestinal epithelia. Trans-epithelial electrical resistance measurements indicate Caco-2 monolayer integrity, and we recorded changes to this integrity following exposure to the fermentation products of selected probiotics and prebiotics, in the form of nondigestible oligosaccharides (NDOs). Our results indicate that NDOs themselves exert varying, but generally minor, effects upon the strength of the tight junctions, whereas the fermentation products of probiotics and NDOs tend to raise tight junction integrity above that of the controls. This effect was bacterial species and oligosaccharide specific. Bifidobacterium Bb 12 was particularly effective, as were the fermentation products of Raftiline and Raftilose. We further investigated the ability of Raftilose fermentations to protect against the negative effects of deoxycholic acid (DCA) upon tight function integrity. We found protection to be species dependent and dependent upon the presence of the fermentation products in the media at the same time as or after exposure to the DCA. Results suggest that the Raftilose fermentation products may prevent disruption of the intestinal epithelial barrier function during damage by tumor promoters.