Protective Effect of Saccharomyces boulardii on Deoxynivalenol-Induced Injury of Porcine Macrophage via Attenuating p38 MAPK Signal Pathway
Protective Effect of Saccharomyces boulardii on Deoxynivalenol-Induced Injury of Porcine Macrophage via Attenuating p38 MAPK Signal Pathway
复制标题
布拉酵母菌通过减弱 P38 Mapk 信号通路对脱氧雪腐镰刀菌烯醇诱导的猪巨噬细胞损伤的保护作用
DOI：
10.1007/s12010-016-2335-x
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发表时间：
2017-05-01
影响因子：
3
通讯作者：
Wu, Jin-E
中科院分区：
文献类型：
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作者：
Chang, Chao;Wang, Kun;Wu, Jin-E
本研究的目的是评价布拉酵母菌（Saccharomyces boulardii，S。目的：探讨DON对猪肺泡巨噬细胞（PAMCs）损伤的保护作用及其机制。3-（4，5-二甲基噻唑-2-基）-2，5-二苯基溴化四氮唑（MTT）试验、流式细胞术分析、ELISA、qRT-PCR和western印迹法用于评估S.布拉氏酵母菌可通过p38丝裂原活化蛋白激酶（p38 MAPK）信号通路保护DON所致的细胞损伤。结果表明，8 μ M DON预处理可降低PAMC的存活率，显著增加PAMC的凋亡率，而S.布拉氏酵母菌能挽救DON诱导的PAMC细胞凋亡。进一步的实验表明，S.布拉地通过下调TNF-α、IL-6和IL-1 β的表达有效地逆转了DON诱导的细胞毒性。此外，S.布拉氏酵母菌能显著降低DON诱导的p38蛋白磷酸化水平和mRNA表达水平，并进一步增加凋亡调控基因Bcl-xl和Bcl-2的表达，抑制Bax的活化。我们的研究结果表明，S.布拉氏酵母菌可抑制DON诱导的PAMC p38 MAPK通路活化，降低下游炎性细胞因子的表达，并促进抗凋亡基因的表达，从而抑制DON诱导的PAMC凋亡。
The aims of our study were to evaluate the effects of Saccharomyces boulardii (S. boulardii) on deoxynivalenol (DON)-induced injury in porcine alveolar macrophage cells (PAMCs) and to explore the underlying mechanisms. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometric analysis, ELISA, qRT-PCR, and western blot were performed to assess whether S. boulardii could prevent DON-induced injury by p38 mitogen-activated protein kinase (p38 MAPK) signal pathway. The results showed that pretreatment with 8 mu M DON could decrease the viability of PAMC and significantly increase the apoptosis rate of PAMC, whereas S. boulardii could rescue apoptotic PAMC cells induced by DON. Further experiments revealed that S. boulardii effectively reversed DON-induced cytotoxicity via downregulating the expression of TNF-alpha, IL-6, and IL-l beta. In addition, S. boulardii significantly alleviated DON-induced phosphorylation and mRNA expression of p38 and further increased the expression of apoptosis regulation genes Bcl-xl and Bcl-2 and inhibited the activation of Bax. Our results suggest that S. boulardii could suppress DON-induced p38 MAPK pathway activation and reduce the expression of downstream inflammatory cytokines, as well as promote the expression of anti-apoptotic genes to inhibit apoptosis induced by DON in PAMC.